Sodium deoxycholate inhibits chick duodenal calcium absorption through oxidative stress and apoptosis

Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
María A RivoiraNori G Tolosa de Talamoni

Abstract

High concentrations of sodium deoxycholate (NaDOC) produce toxic effects. This study explores the effect of a single high concentration of NaDOC on the intestinal Ca(2+) absorption and the underlying mechanisms. Chicks were divided into two groups: 1) controls and 2) treated with different concentrations of NaDOC in the duodenal loop for variable times. Intestinal Ca(2+) absorption was measured as well as the gene and protein expressions of molecules involved in the Ca(2+) transcellular pathway. NaDOC inhibited the intestinal Ca(2+) absorption, which was concentration dependent. Ca(2+)-ATPase mRNA decreased by the bile salt and the same occurred with the protein expression of Ca(2+)-ATPase, calbindin D(28k) and Na(+)/Ca(2+) exchanger. NaDOC produced oxidative stress as judged by ROS generation, mitochondrial swelling and glutathione depletion. Furthermore, the antioxidant quercetin blocked the inhibitory effect of NaDOC on the intestinal Ca(2+) absorption. Apoptosis was also triggered by the bile salt, as indicated by the TUNEL staining and the cytochrome c release from the mitochondria. As a compensatory mechanism, enzyme activities of the antioxidant system were all increased. In conclusion, a single high concentration of NaD...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jul 12, 1973·Biochimica Et Biophysica Acta·C O Beauchamp, I Fridovich
Dec 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S KrähenbühlJ Reichen
Oct 1, 1996·Pediatric Research·J P ButsE M Sokal
Oct 1, 1996·Comparative Biochemistry and Physiology. Part A, Physiology·N Tolosa de TalamoniA Alisio
Apr 14, 2000·Pflügers Archiv : European journal of physiology·A C MauricioR Greger
Oct 31, 2000·Critical Reviews in Toxicology·J D Robertson, S Orrenius
Jan 26, 2002·Medical Electron Microscopy : Official Journal of the Clinical Electron Microscopy Society of Japan·M Oumi, T Yamamoto
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 2, 2003·The Journal of Nutritional Biochemistry·Ana M MarchionattiNori G Tolosa de Talamoni
Nov 6, 2004·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Viviana A CentenoNori G Tolosa de Talamoni
Jan 18, 2005·Mutation Research·H BernsteinH Garewal
Dec 3, 2005·Cell Biology and Toxicology·C M PayneH Garewal
Mar 21, 2006·The Journal of Biological Chemistry·Samira Jean-LouisJesse D Martinez
Nov 1, 1965·The Biochemical Journal·D D Webling, E S Holdsworth
Dec 7, 2007·Biochimica Et Biophysica Acta·Ana M MarchionattiNori G Tolosa de Talamoni
Oct 7, 2008·World Journal of Gastroenterology : WJG·Olga Martinez-Augustin, Fermin Sanchez de Medina
Apr 29, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Heping YangShelly C Lu
Jul 18, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Akira MiyakiAndrew J Dannenberg
Dec 15, 2010·Journal of the Korean Society of Coloproctology·Yun-Hyung Ha, Dong-Guk Park
Jan 27, 2011·Archives of Toxicology·Carol BernsteinHarris Bernstein

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Citations

Dec 29, 2015·Archives of Biochemistry and Biophysics·Valeria A RodríguezNori G Tolosa de Talamoni
Oct 16, 2015·Nutrition Research Reviews·Vanessa ArecoNori Tolosa de Talamoni
Mar 21, 2017·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Ana M MarchionattiNori G Tolosa de Talamoni
Jun 22, 2019·The Journal of Physiological Sciences : JPS·Kannikar WongdeeNarattaphol Charoenphandhu
May 20, 2017·World Journal of Gastroenterology : WJG·Gabriela Diaz de BarbozaNori Tolosa de Talamoni
Dec 5, 2018·World Journal of Gastroenterology : WJG·Luciana MoineNori Tolosa de Talamoni
Jul 14, 2020·World Journal of Gastroenterology : WJG·Vanessa A ArecoMaría E Peralta López
Jul 21, 2020·Canadian Journal of Physiology and Pharmacology·Adriana PérezNori Tolosa de Talamoni

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