PMID: 3929619Sep 1, 1985

Sodium dependence of luminal alkalinization by rabbit ileal mucosa

The American Journal of Physiology
P L SmithS K Sullivan

Abstract

Stripped rabbit ileal mucosa was studied in vitro in Ussing chambers under short-circuit conditions using the pH-stat technique to determine basal rates of luminal alkalinization; the contribution of the shunt pathway to the alkalinization process; the effects of Na, Cl, or HCO3 removal from the bathing solutions on luminal alkalinization; and the effects of epinephrine, ouabain, 4,4'-diisothiocyanostilbene-2,2'-disulfonate (DIDS), acetazolamide, prostaglandin E1 (PGE1), A23187, sugars, or amino acids on the alkalinization process. Results from these studies reveal that, under basal conditions, the rate of luminal alkalinization accounts for 81% of the basal short-circuit current (Isc), although there was no correlation between the rate of alkalinization and Isc. The contribution of the shunt to the alkalinization process accounts for less than 10% of the mucosal-to-serosal HCO3 flux. Removal of Cl from the bathing solutions increased the rate of luminal alkalinization and decreased Isc. Sodium removal from the bathing solutions reduced both Isc and the rate of luminal alkalinization. Addition of DIDS to the luminal or serosal bathing solution reduced luminal alkalinization less than 30%. Acetazolamide, PGE1, and A23187 were al...Continue Reading

Citations

Aug 17, 2004·American Journal of Physiology. Cell Physiology·Sadasivan VidyasagarHenry J Binder
Jan 29, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Sadasivan VidyasagarVazhaikkurichi M Rajendran
Sep 27, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·B R GrubbR C Boucher

Related Concepts

Calcimycin
Acetazolamide Sodium, (Sterile)
Caverject
Medihaler-Epi
Hydrogen-Ion Concentration
Ileum
Structure of Intestinal Gland
Ouabain
Prostaglandins E
4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid, Disodium Salt

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