Sodium dependence of lysophosphatidylcholine-induced caspase-1 activity and reactive oxygen species generation

Immunobiology
Tom Schilling, Claudia Eder

Abstract

The proinflammatory cytokines interleukin (IL)-1β and IL-18 play pivotal roles in neuroinflammatory diseases. Caspase-1-mediated proteolytic cleavage is required to convert the premature, biologically inactive cytokines to their biologically active forms capable of promoting tissue inflammation. Although caspases have been recognized as potential therapeutic targets in inflammatory diseases, mechanisms regulating caspase-1 activation are not fully understood. Here we demonstrate that the proinflammatory lipid lysophosphatidylcholine (LPC) initiates microglial caspase-1 activation in a Na(+)-dependent manner. LPC-induced caspase-1 activity was almost completely inhibited upon omission of extracellular Na(+), but was unaffected by inhibition of Na(+)/K(+)-ATPase with ouabain or by inhibition of Na(+)/H(+) antiport with amiloride. Inhibition of caspase-1-mediated IL-1β processing by Na(+)-free medium led to reduced amounts of mature IL-1β released from LPC-stimulated microglia. Furthermore, LPC-induced production of reactive oxygen species (ROS) was abolished by Na(+)-free medium, indicating Na(+) dependence of NADPH oxidase activity in LPC-stimulated microglia. Since ROS production was found to be crucial to caspase-1 activation ...Continue Reading

References

Apr 1, 1992·Journal of Neuroscience Research·V BocchiniH Kettenmann
May 1, 1990·Journal of Neuroimmunology·E BlasiF Bistoni
Oct 1, 1984·Journal of Neurochemistry·G Y Sun, L L Foudin
Nov 22, 1996·The Journal of Biological Chemistry·D G PerregauxC A Gabel
Jan 14, 1998·Brain Research. Molecular Brain Research·J Yao, R W Johnson
Feb 11, 1998·Physiological Reviews·F LangD Häussinger
Dec 9, 1998·The American Journal of Physiology·D G Perregaux, C A Gabel
May 9, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·I WalevS Bhakdi
Mar 17, 2004·The Journal of Physiology·Tom SchillingClaudia Eder
Apr 7, 2004·The European Journal of Neuroscience·Tom SchillingClaudia Eder
Jul 19, 2005·Trends in Neurosciences·Ursula Felderhoff-MueserPhilip F Stahel
Jul 22, 2005·Nature Reviews. Immunology·Stuart M AllanNancy J Rothwell
Oct 4, 2006·Physiological Reviews·Michael J Page, Enrico Di Cera
Dec 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christian StockClaudia Eder
Dec 21, 2006·Nature Reviews. Neuroscience·Michelle L BlockJau-Shyong Hong
May 17, 2007·Epilepsy Currents·Annamaria Vezzani, Tallie Z Baram
Feb 27, 2008·Journal of Cellular and Molecular Medicine·B Howley, H O Fearnhead
Jun 24, 2008·Annals of Neurology·Florian S EichlerRichard M Ransohoff
Oct 3, 2008·Biological Research for Nursing·Alice E PasvogelIda M Ki Moore
Nov 14, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Cyril ChéretMichel Mallat
Jan 6, 2009·BMC Neuroscience·Michelle L Block
Dec 31, 2008·Glia·Abdullah Md SheikhJunichi Masuda
Mar 3, 2009·Immunobiology·Claudia Eder
Mar 24, 2009·Annual Review of Immunology·Fabio MartinonJürg Tschopp
Mar 24, 2009·Annual Review of Immunology·Charles A Dinarello
Jun 30, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sara TassiAnna Rubartelli
Aug 18, 2009·Journal of Neuroimmunology·Tom Schilling, Claudia Eder
Feb 4, 2010·Journal of Neuroscience Research·Swarupa ChakrabortyAnirban Basu
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Frank L van de VeerdonkMihai G Netea
Feb 20, 2010·Nature Reviews. Immunology·Jurg Tschopp, Kate Schroder

❮ Previous
Next ❯

Citations

Mar 4, 2014·Progress in Neurobiology·Jason R PlemelWolfram Tetzlaff
Jan 7, 2015·International Journal of Molecular Sciences·Chunfa Huang, Carl Freter
Apr 3, 2012·Experimental Neurology·Damien P Kuffler
Jun 18, 2020·International Journal of Molecular Sciences·Kelvin Ka-Lok WuKenneth King-Yip Cheng
Feb 23, 2020·Life Sciences·Panpan LiuCong Peng
Jul 7, 2021·Antimicrobial Agents and Chemotherapy·Maizbha U AhmedJian Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

Caspases in Metabolic Diseases

Caspases, the family of cysteine proteases are involved in programmed cell death, but their role in metabolic diseases, inflammation and immunity has been of interested. Discover the latest research on caspases in metabolic diseases here.