Sodium dodecyl sulphate-protein complexes. Changes in size or shape below the critical micelle concentration, as monitored by high-performance agarose gel chromatography

Journal of Chromatography
E Mascher, P Lundahl

Abstract

We have determined the sodium dodecyl sulphate (SDS) concentration needed to complete the formation of SDS-protein complexes. A Superose-6 column was equilibrated with SDS for 7 h. A sample of a native protein or an SDS-protein complex was applied, and the elution volume, Ve, was determined. Then the SDS concentration, CSDS, was changed, etc., i.e., Ve was determined as a function of CSDS. The critical micelle concentration of SDS (cmcSDS) was 1.8 mM in the eluent (ionic strength 0.10 M). Native bovine carbonic anhydrase (BCA) formed an SDS complex above 0.2 mM SDS. As CSDS was increased, Ve decreased gradually in two main transitions, (TI) at 0.2-1.0 mM and (TII) at 1.2-2.0 mM SDS. These concentrations are corrected for a lag in the column equilibration with SDS. SDS-BCA, pre-equilibrated at 1.6 mM SDS, showed transitions similar to those observed with native BCA, except that transition TII included a minor transition at 2.0-2.2 mM SDS. The SDS complexes of reduced and carboxamidomethylated bovine serum albumin, of N-5'-phosphoribosylanthranilate isomerase-indole-3-glycerol-phosphate synthase from Escherichia coli (PRAI-IGPS) and of two tryptic fragments of this enzyme behaved similarly. For SDS-PRAI-IGPS the major part of tra...Continue Reading

References

Jan 1, 1979·Methods in Enzymology·A HeleniusC Tanford
Sep 1, 1979·Journal of Biochemistry·T ImamuraK Konishi
Jan 1, 1978·Journal of Supramolecular Structure·D C Sogin, P C Hinkle
Feb 21, 1977·Journal of Chromatography·R J Blagrove, M J Frenkel
Jun 1, 1988·Trends in Biochemical Sciences·A R Walmsley
Jul 1, 1988·Molecular and Cellular Biochemistry·R E Abbott, D Schachter
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J P PriestleJ N Jansonius
Sep 6, 1985·Science·M MuecklerH F Lodish
Oct 1, 1968·The Biochemical Journal·R Pitt-Rivers, F S Impiombato
Sep 7, 1967·Biochemical and Biophysical Research Communications·A L ShapiroJ V Maizel
Jul 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·J A Reynolds, C Tanford
Jan 25, 1984·Biochimica Et Biophysica Acta·G E LienhardK J Ransome

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Citations

Sep 24, 1999·Protein Science : a Publication of the Protein Society·D E OtzenM Schülein
Feb 6, 2002·Journal of Molecular Biology·Daniel E Otzen, Mikael Oliveberg
Jun 26, 2010·Biochemistry·Arpana DuttaJudith Klein-Seetharaman

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