Abstract
Many gram-negative bacteria are known to be remarkably resistant to hydrophobic noxious agents by virtue of their outer membranes (OM). We investigated, by using four different assay methods, the ability of sodium hexametaphosphate (HMP) to disrupt this OM barrier. (i) In the growth inhibition assay, HMP was found to sensitize strains of Pseudomonas aeruginosa to all the hydrophobic probes tested (rifampin, fusidic acid, dactinomycin, sodium dodecyl sulfate, and Triton X-100). A concentration of 0.3% HMP decreased the MICs of the probes by a factor of approximately 10, and maximally even a 30-fold sensitization was found with 1% HMP. (ii) In the bactericidal assay, 0.3% HMP decreased the MBC of the hydrophobic probe rifampin by a factor of approximately 30. (iii) In the bacteriolytic assay, 0.1% HMP sensitized the target bacteria to lysis by sodium dodecyl sulfate and Triton X-100. (iv) In the fluorescent-probe binding assay, HMP drastically enhanced the binding of fluorescent N-phenyl naphthylamine to the membranes of the target cells. In addition to P. aeruginosa, P. fluorescens, P. putida, P. fragi, and Escherichia coli were susceptible to the OM permeability-increasing action of HMP, while P. cepacia was resistant.
References
Oct 1, 1978·Journal of Bacteriology·R E Hancock, H Nikaido
Mar 1, 1985·Microbiological Reviews·H Nikaido, M Vaara
Jun 10, 1988·Journal of Chromatography·M Vaara
Apr 1, 1985·Antimicrobial Agents and Chemotherapy·M Vaara, P Viljanen
Dec 1, 1986·Antimicrobial Agents and Chemotherapy·R A Moore, R E Hancock
Feb 3, 1986·European Journal of Biochemistry·R HukariM Vaara
Jul 1, 1986·The Journal of Infectious Diseases·H R Knapp, M A Melly
Sep 1, 1988·Infection and Immunity·P ViljanenM Vaara
Jul 1, 1988·Journal of Bacteriology·S Wee, B J Wilkinson
May 10, 1974·Annals of the New York Academy of Sciences·L Leive
Jul 1, 1983·Antimicrobial Agents and Chemotherapy·M Vaara, T Vaara
Mar 1, 1983·Infection and Immunity·M A PurdyW E White
Jun 9, 1983·Nature·M Vaara, T Vaara
Jul 1, 1983·Antimicrobial Agents and Chemotherapy·M Vaara, T Vaara
Jul 1, 1984·Antimicrobial Agents and Chemotherapy·R E Hancock, P G Wong
Nov 1, 1981·Journal of Bacteriology·M Vaara
Feb 1, 1982·Journal of Bacteriology·Y Uratani
Jun 1, 1980·Journal of Bacteriology·E S Tecoma, D Wu
Sep 1, 1963·Applied Microbiology·F J POSTM D FLANAGAN
Citations
Feb 6, 2004·FEMS Microbiology Letters·R A DixonG Alderson
Oct 21, 1999·International Journal of Antimicrobial Agents·P Collignon, J Turnidge
Mar 29, 2000·Journal of Applied Microbiology·I M Helander, T Mattila-Sandholm
Aug 30, 2011·The Journal of Antimicrobial Chemotherapy·Gavin HumphreysAndrew J McBain
Feb 21, 2008·Antimicrobial Agents and Chemotherapy·Hayssam KhalilZhao Wang
May 1, 1990·Antimicrobial Agents and Chemotherapy·M Vaara
Apr 1, 1992·Antimicrobial Agents and Chemotherapy·R Vuorio, M Vaara
Nov 19, 2008·BMC Microbiology·Susana Sánchez-GómezGuillermo Martínez-de-Tejada
Jul 17, 2012·Biochemical and Biophysical Research Communications·Rebekah P NashMatthew R Redinbo
Jan 25, 2006·Journal of Applied Microbiology·A A Al-Nabulsi, R A Holley
Oct 1, 2010·Journal of Applied Microbiology·A BaumstummlerS Ribault
Dec 18, 2013·Journal of Dentistry·Danielle Mendes da CamaraAlberto Carlos Botazzo Delbem
Nov 9, 2002·Folia Microbiologica·A GuhaM K Roy
Aug 23, 2016·Journal of Microbiological Methods·Erin E SilvestriFrank W Schaefer
Feb 25, 1999·Letters in Applied Microbiology·H M AyresA D Russell
Sep 15, 1999·Anesthesiology·P PereM Vaara
Feb 28, 2018·Biofouling·Carla Corrêa Mendes-GouvêaDebora Barros Barbosa
Dec 13, 2018·Clinical Oral Investigations·Luhana Santos Gonzales GarciaMarcelle Danelon
Oct 6, 2000·Microbiology·L GoodC Wahlestedt
Jan 9, 1999·Clinical Microbiology Reviews·G McDonnell, A D Russell
Sep 1, 1992·Microbiological Reviews·M Vaara
Aug 1, 1996·Antimicrobial Agents and Chemotherapy·M Vaara, M Porro
May 23, 2000·Journal of Agricultural and Food Chemistry·M A Sattar KhanA Kato