Sodium nitroprusside stimulates L-DOPA release from striatal tissue through nitric oxide and cGMP

European Journal of Pharmacology
Juan J SanchezM Carmen Gonzalez

Abstract

The effects of the nitric oxide (NO) donor, sodium nitroprusside, on L-DOPA and dopamine release from striatal tissue were evaluated using a static incubation system in which the striatal tissue released between three and six times more L-DOPA than DA, although the DA content was four times higher than that of L-DOPA. Sodium nitroprusside stimulated L-DOPA release in a time- and concentration-dependent (25, 50 and 100 microM) manner. This effect was not due to an increase in L-DOPA synthesis because sodium nitroprusside did not modify the tyrosine hydroxylase activity of striatal tissue. DA release was also stimulated by sodium nitroprusside but it required a higher concentration (500 microM) and longer incubation (60 min). Neither basal nor sodium nitroprusside-stimulated L-DOPA release was influenced by Ca(2+) deprivation (EGTA 5 mM) and/or the presence of nitrendipine (1 microM), a blocker Ca(2+) channel, in the incubation medium. However, cGMP (1 mM) increased L-DOPA release, and the soluble guanylate cyclase inhibitor, 1H-[1,2,4]oxadiazolo[4,3-alpha]quinoxalin-1-one (ODQ) (5 microM), partially blunted the stimulatory effect of sodium nitroprusside 100 microM. In addition, the presence of certain scavengers of free radicals...Continue Reading

References

Feb 15, 1991·Biochemical Pharmacology·L J Ignarro
Feb 5, 1990·Brain Research·G L Snyder, M J Zigmond
Jan 1, 1993·Free Radical Research Communications·R E Huie, S Padmaja
Aug 1, 1996·Progress in Neurobiology·Y MisuH Okamura
Nov 10, 2000·Life Sciences·P AbreuM C González

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Citations

Jun 6, 2006·European Journal of Pharmacology·P Abreu-GonzálezM C González
Dec 3, 2014·Neurobiology of Learning and Memory·P R A HeckmanJ Prickaerts
Dec 14, 2017·Expert Opinion on Drug Metabolism & Toxicology·Hojin Choi, Seong-Ho Koh
Feb 12, 2020·Neuroscience and Biobehavioral Reviews·Elentina K ArgyrousiJos Prickaerts

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