Sodium selenite suppresses hepatitis B virus transcription and replication in human hepatoma cell lines.

Journal of Medical Virology
Zhikui ChengKanghong Hu

Abstract

Hepatitis B virus (HBV) infection is one of the most serious and prevalent health problems worldwide. Current anti-HBV medications have a number of drawbacks, such as adverse effects and drug resistance; thus, novel potential anti-HBV reagents are needed. Selenium (Se) has been shown to be involved in both human immunodeficiency virus and hepatitis C virus infections, but its role in HBV infection remains unclear. To address this, sodium selenite (Na2SeO3 ) was applied to three HBV cell models: HepG2.2.15 cells, and HuH-7 cells transfected with either 1.1 or 1.3× HBV plasmids. Cytotoxicity of Na2SeO3 was examined by Cell Counting Kit-8. Levels of viral antigen expression, transcripts, and encapsidated viral DNA were measured by enzyme-linked immunosorbent assay, northern blot, and Southern blot, respectively. There was no obvious cytotoxicity in either HepG2.2.15 or HuH-7 cells with <2.5 µM Na2SeO3 . Below this concentration, Na2SeO3 suppressed HBsAg and HBeAg production, HBV transcript level, and amount of genomic DNA in all three tested models, and suppression level was enhanced in line with increases in Na2 SeO3 concentration or treatment time. Moreover, the inhibitory effect of Na2SeO3 on HBV replication can be further enha...Continue Reading

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Citations

Sep 7, 2019·Nutrients·Olivia M GuillinLaurent Chavatte
Aug 20, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Jiayu ZhongHong Cao
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Jan 10, 2021·Bulletin of Environmental Contamination and Toxicology·Qizhuang LvYulin Zhu
Apr 10, 2021·Current Nutrition Reports·Saroj Khatiwada, Astha Subedi
Jun 28, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Akbar HasanzadehMichael R Hamblin
Aug 17, 2021·Frontiers in Pharmacology·Wensen LinJiang Pi

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Datasets Mentioned

BETA
U95551.1

Methods Mentioned

BETA
Assay
ELISA

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