PMID: 9543417Apr 16, 1998Paper

Sodium TQF NMR and intracellular sodium in isolated crystalloid perfused rat heart

Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine
Victor D SchepkinJ N Young

Abstract

The feasibility of monitoring intracellular sodium changes using Na triple quantum filtered NMR without a chemical shift reagent (SR) was investigated in an isolated rat heart during a variety of interventions for Na(i) loading. Perfusion with 1 mM ouabain or without K+ present in the perfusate for 30 min produced a rise of the Na TQF signal with a plateau of approximately 190% and approximately 228% relative to the preintervention level, respectively. Stop-flow ischemia for 30 min resulted in a TQF signal growth of approximately 147%. The maximal Na TQF signal increase of 460% was achieved by perfusion without K+/Ca2+, corresponding to an elimination of the Na transmembrane gradient. The observed values of Na NMR TQF growth in the physiological and pathological ranges are in agreement with reported data by other methods and have a linear correlation with intracellular sodium content as determined in this study by Co-EDTA method and by sucrose-histidine washout of the extracellular space. Our data indicate that the increase in Na TQF NMR signal is determined by the growth of Na(i), and the extracellular Na contribution to the total TQF signal is unchanged at approximately 64%. In conclusion, Na TQF NMR without using SR offers a...Continue Reading

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Citations

Jun 17, 1999·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·J M ColetA D Sherry
Sep 29, 1999·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·S E AndersonP M Cala
Jun 5, 2003·The Journal of Clinical Investigation·John R Forder, Gerald M Pohost
Jan 23, 2014·Magma·Simon Konstandin, Lothar R Schad
May 13, 2014·Progress in Nuclear Magnetic Resonance Spectroscopy·Guillaume MadelinAlexej Jerschow
Sep 2, 1999·Journal of Molecular and Cellular Cardiology·V D SchepkinW M DeCampli
May 3, 2005·Journal of Magnetic Resonance·Costin Tanase, Fernando E Boada
Jul 14, 2020·NMR in Biomedicine·Dennis KleimaierLothar R Schad
Jul 5, 2018·Biochemical Society Transactions·Dunja AksentijevicMichael J Shattock
Oct 14, 2020·Journal of Neurology·Teresa GerhalterMatthias Türk
Jun 5, 2019·Zeitschrift für medizinische Physik·Victor D Schepkin
Mar 18, 2017·Journal of Magnetic Resonance·Victor D SchepkinThomas F Budinger
May 1, 2021·Brain Communications·Teresa GerhalterGuillaume Madelin
Aug 10, 2021·Journal of Molecular and Cellular Cardiology·Dunja Aksentijević, Michael J Shattock

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