PMID: 8443224Feb 23, 1993Paper

Sodium transport by an ionizable and a neutral mobile carrier: effects of membrane structure on the apparent activation energy

Biochimica Et Biophysica Acta
G VareilleM Castaing

Abstract

Temperature-jump relaxation experiments on Na+ transport by (221)C10-cryptand (ionizable mobile carrier) and nonactin (neutral mobile carrier) were carried out in order to study the effects of cholesterol and the degree of acyl chain unsaturation, and their temperature-dependence on ion transport through thin lipid membranes. The experiments were performed on large, negatively charged unilamellar vesicles (LUV) prepared from mixtures of phosphatidylcholine (egg phosphatidylcholine, dioleoylphosphatidylcholine and dilinoleolylphosphatidylcholine), phosphatidic acid and cholesterol (mole fractions 0-0.43), at various temperatures and carrier concentrations. The apparent rate constants of Na+ translocation by (221)C10 and nonactin increased with the carrier concentration, the degree of acyl chain unsaturation and the temperature. The incorporation of cholesterol into the membranes significantly reduced the carrier concentration-, acyl chain unsaturation- and temperature-dependence of this parameter. The apparent energy required to activate the transport decreased significantly with increasing (221)C10 concentrations and remained constant with increasing those of nonactin at any given cholesterol molar fraction and degree of acyl c...Continue Reading

References

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Citations

May 4, 2004·Journal of Colloid and Interface Science·Lingyun Zhao, Si-Shen Feng
Aug 3, 1994·Biochimica Et Biophysica Acta·G MulliertM Castaing

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