PMID: 702522Sep 25, 1978Paper

Sodium uptake across the apical border of the isolated turtle colon: confirmation of the two-barrier model

The Journal of Membrane Biology
S M Thompson, D C Dawson

Abstract

The initial rate of Na uptake by the turtle colon from the mucosal bathing solution consists of two operationally distinct components. One component is a linear function of mucosal Na concentration, is unaffected by amiloride, and appears to represent Na uptake into the paracellular shunt path. The major component of Na uptake is abolished by amiloride and is virtually equal to the short-circuit current over a wide range of mucosal Na concentrations, suggesting that this portion of Na uptake represents Na movement into Na-transporting cells of the colon. The amiloride-sensitive component of Na uptake, at low mucosal Na concentrations, was unaffected if net Na transport was abolished by ouabain. Similarly, at low mucosal Na concentrations the amiloride-sensitive conductance of the colon was identical in the presence and in the absence of net Na transport. These results show that the isolated turtle colon behaves as two distinct barriers to transmural Na transport, an apical barrier blocked by amiloride and a more basal-lying barrier where active, transmural Na transport is blocked by ouabain. In addition, these experiments appear to provide the first unambiguous demonstration that the initial-rate isotope uptake technique can pr...Continue Reading

References

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Citations

Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·G J LegrisU Hopfer
Sep 1, 1983·The Journal of General Physiology·K L Kirk, D C Dawson
Oct 1, 1983·The Journal of General Physiology·K L Kirk, D C Dawson
Aug 1, 1986·The Journal of General Physiology·W J GermannD C Dawson
Nov 1, 1982·Kidney International·J B Stokes
Jul 1, 1984·The American Journal of Physiology·D R Halm, D C Dawson
Jul 1, 1986·The American Journal of Physiology·N W Richards, D C Dawson
Nov 1, 1985·The American Journal of Physiology·S M O'GradyM C Rao
Oct 1, 1986·The American Journal of Physiology·C J Venglarik, D C Dawson
Nov 1, 1985·The American Journal of Physiology·J AhnM Field

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