Soft landing of cell-sized vesicles on solid surfaces for robust vehicle capture/release

Soft Matter
Dehui WangPeng Yang

Abstract

Based on a concept of a smooth and steady landing of fragile objects without destruction via a soft cushion, we have developed a model for the soft landing of deformable lipid giant unilamellar vesicles (GUVs) on solid surfaces. The foundation for a successful soft landing is a solid substrate with a two-layer coating, including a bottom layer of positively charged lysozymes and an upper lipid membrane layer. We came to a clear conclusion that anionic GUVs when sedimented on a surface, the vesicle rupture occurs upon the direct contact with the positively charged lysozyme layer due to the strong coulombic interactions. In contrast, certain separation distances was achieved by the insertion of a soft lipid membrane cushion between the charged GUVs and the lysozyme layer, which attenuated the coulombic force and created a mild buffer zone, ensuring the robust capture of GUVs on the substrate without their rupture. The non-covalent bonding facilitated a fully reversible stimuli-responsive capture/release of GUVs from the biomimetic solid surface, which has never been demonstrated before due to the extreme fragility of GUVs. Moreover, the controllable capture/release of cells has been proven to be of vital importance in biotechnolo...Continue Reading

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Citations

Dec 17, 2016·ACS Applied Materials & Interfaces·Jin GuPeng Yang
Feb 13, 2018·Biomaterials Science·Chen LiPeng Yang
Jul 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jian ZhaoPeng Yang
Dec 18, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Fei TaoPeng Yang

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