Soft mechano-chemistry of molecular hubs in mitotic spindle: biomechanics and mechanical proofreading at microtubule ends.

Journal of Physics. Condensed Matter : an Institute of Physics Journal
Debashish Chowdhury, Dipanwita Ghanti

Abstract

A microtubule (MT) is a long stiff tube-shaped filament formed by a hierarchical organization of a large number of tubulin protein molecules. These filaments constitute a major structural component of the scaffold of a multi-component macromolecular machine called mitotic spindle. The plus ends of the MTs are tethered to some specific binding partners by molecular tethers while those of some others are crosslinked by crosslinking molecules. Because of the non-covalent binding involved in the tethering and crosslinking, the attachments formed are intrinsically 'soft'. These attachments are transient because these can get ruptured spontaneously by thermal fluctuations. By implementing in silico the standard protocols of in vitro molecular force spectroscopy, we compute the lifetimes of simple theoretical models of these attachments. The mean lifetime is essentially a mean first-passage time. The stability of cross-linked antiparallel MTs is shown to decrease monotonically with increasing tension, a characteristic of all 'slip-bonds'. This is in sharp contrast to the nonmonotonic variation of the mean lifetime with tension, a mechanical fingerprint of 'catch-bonds', displayed by the MTs tethered to two distinct binding partners. W...Continue Reading

References

Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T L Hill
Jan 31, 1997·Science·R B Nicklas
May 8, 2001·Annual Review of Biophysics and Biomolecular Structure·E Evans
Oct 20, 2001·Science·E Karsenti, I Vernos
Jun 25, 2002·Biophysical Journal·Ajit P Joglekar, Alan J Hunt
Aug 12, 2005·Chemical Reviews·Martin K Beyer, Hauke Clausen-Schaumann
Oct 14, 2006·Journal of Cell Science·Francesca Bartolini, Gregg G Gundersen
Dec 19, 2006·Cell Division·Megan T ValentineSteven M Block
Mar 17, 2007·Annual Review of Biochemistry·Stefan WestermannGeorjana Barnes
Apr 12, 2007·Nature Reviews. Molecular Cell Biology·Andrea Musacchio, Edward D Salmon
Sep 4, 2007·Trends in Cell Biology·Trisha N Davis, Linda Wordeman
Nov 22, 2007·Proceedings of the National Academy of Sciences of the United States of America·Artem EfremovFazly I Ataullakhanov
Mar 19, 2008·Proceedings of the National Academy of Sciences of the United States of America·Melanie J I MüllerReinhard Lipowsky
Jun 25, 2008·Annual Review of Biophysics·Wendy E ThomasEvgeni Sokurenko
Jul 24, 2008·Annual Review of Biomedical Engineering·Wendy Thomas
Oct 16, 2008·Cell Host & Microbe·Evgeni V SokurenkoWendy E Thomas
Nov 4, 2008·Current Biology : CB·Lukas C KapiteinErwin J G Peterman
Apr 1, 2009·Accounts of Chemical Research·Oleg V Prezhdo, Yuriy V Pereverzev
Feb 13, 2010·Journal of Molecular Recognition : JMR·Martin Karplus
May 15, 2010·Methods in Cell Biology·Leonid A Mirny, Daniel J Needleman
Oct 12, 2010·Journal of Cell Science·Alex Mogilner, Erin Craig
Oct 19, 2010·Trends in Cell Biology·Charles L AsburyTrisha N Davis
Jan 29, 2011·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Graham J Buttrick, Jonathan B A Millar
Sep 6, 2011·Nature Cell Biology·Marcus BraunMarcel E Janson
Feb 1, 2012·Chromosoma·Bungo Akiyoshi, Sue Biggins
Feb 11, 2012·Quarterly Reviews of Biophysics·J Richard McIntoshFazly I Ataullakhanov
Aug 8, 2012·Proceedings of the National Academy of Sciences of the United States of America·Raymond W FriddleJames J De Yoreo
Nov 17, 2012·Developmental Cell·Radhika Subramanian, Tarun M Kapoor
Dec 22, 2012·Nature Reviews. Molecular Cell Biology·Emily A Foley, Tarun M Kapoor
Jan 5, 2013·Current Opinion in Cell Biology·Marileen Dogterom, Thomas Surrey
Jan 25, 2013·Chemical Society Reviews·Laszlo Takacs
Feb 20, 2013·The Journal of Cell Biology·J Richard McIntoshEkaterina L Grishchuk
Mar 28, 2013·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Hugo Bowne-AndersonJonathon Howard
Nov 22, 2013·Biochemical Society Transactions·Jonathan L D Lawson, Rafael E Carazo Salas
Mar 19, 2014·Trends in Genetics : TIG·Krishna K Sarangapani, Charles L Asbury
Jul 6, 2014·Cold Spring Harbor Perspectives in Biology·Iain M Cheeseman
Nov 5, 2014·Molecular Biology of the Cell·Sophie Dumont, Manu Prakash
Nov 15, 2014·Molecular Biology of the Cell·Erik C Yusko, Charles L Asbury
May 10, 2015·The Journal of Biological Chemistry·Ray Alfaro-Aco, Sabine Petry

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