Sohlh2 inhibits the apoptosis of mouse primordial follicle oocytes via C-kit/PI3K/Akt/Foxo3a signalling pathway

Reproductive Biomedicine Online
Xiaoli ZhangJing Hao

Abstract

We previously reported that bone morphogenetic protein 4/drosophila mothers against decapentaplegic protein (BMP4/Smad) signalling pathway initiated primordial follicle growth and prevented oocyte apoptosis via up-regulation of Sohlh2 and receptor for kit ligand (c-kit). The mechanism underlying this process was not fully elucidated. In the present study, primary oocyte cultures were established from ovaries of 3-day-old female mouse pups by two-step enzyme digestion. Cultures were divided into Sohlh2 small interference RNA (SiRNA) group, negative SiRNA group, Sohlh2 overexpression plasmid group and pCAG-puro group. TdT (terminal deoxynucleotidyl transferase)-mediated dUDP nick-end labelling assay was carried out to detect the oocyte apoptosis; immunocytochemical staining and quantitative real time-polymerase chain reaction detected the expression of c-kit and Forkhead box O3a (Foxo3a); Western blot was performed to detect the expression of Sohlh2, C-kit, saerine/threonine kinases (Akt1) and Foxo3a. The results showed that Sohlh2 inhibited oocyte apoptosis and upregulated c-kit expression; Sohlh2 decreased the endonuclear Foxo3a via the upregulation of phosphorylated Akt1 (P-Akt1) and phosphorylated Foxo3a (P-Foxo3a) but not to...Continue Reading

References

Mar 7, 2003·Science's STKE : Signal Transduction Knowledge Environment·Hien TranMichael E Greenberg
Jul 12, 2003·Science·Diego H CastrillonRonald A DePinho
Feb 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Taisuke HosakaKaren C Arden
Apr 13, 2004·Proceedings of the National Academy of Sciences of the United States of America·Xiaoxia QiGuang-Quan Zhao
Oct 30, 2004·Molecular Reproduction and Development·Xuan JinYi-Xun Liu
Jan 20, 2005·The Journal of Biological Chemistry·Marc D JacobsKumkum Saxena
Nov 19, 2008·Developmental Biology·Shuichi ToyodaJun-ichi Miyazaki
Mar 5, 2009·Biochemical and Biophysical Research Communications·Hong LiuRen-Li Zhang
Aug 23, 2011·Molecular and Cellular Endocrinology·Krishna Jagarlamudi, Aleksandar Rajkovic
Nov 8, 2011·Developmental Biology·Hitomi SuzukiAleksandar Rajkovic
Feb 14, 2012·Journal of Cell Science·Florencia BarriosSusanna Dolci
Jan 25, 2013·Clinical and Experimental Reproductive Medicine·Eun-Jin Lim, Youngsok Choi
Jan 25, 2014·Environmental and Molecular Mutagenesis·Teng ZhangWei Shen
Feb 15, 2014·Fertility and Sterility·Yingying QinZi-Jiang Chen

❮ Previous
Next ❯

Citations

Oct 19, 2016·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Marco D'AuroraValentina Gatta
Feb 26, 2016·Biology of Reproduction·Yanzhou YangYanrong Wang
Oct 26, 2018·Molecular Reproduction and Development·Gongqing LiuZhihong Zheng
Dec 15, 2018·Human Reproduction·Christiani A AmorimMarie-Madeleine Dolmans
Aug 8, 2020·Endocrine Reviews·Johanne GrosboisIsabelle Demeestere
Sep 12, 2019·Reproductive Biology and Endocrinology : RB&E·Jun TanQiong-Fang Wu
Feb 16, 2017·Cellular and Molecular Life Sciences : CMLS·Chao WangGuoliang Xia
May 10, 2021·Reproductive Sciences·Dongyong YangYanxiang Cheng

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

B-Cell Lymphoma

B-cell lymphomas include lymphomas that affect B cells. This subtype of cancer accounts for over 80% of non-Hodgkin lymphomas in the US. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis