Soil Bioassay for Detecting Magnaporthiopsis maydis Infestation Using a Hyper Susceptible Maize Hybrid

Journal of Fungi
Ofir DeganiOnn Rabinovitz

Abstract

Magnaporthiopsis maydis is the causal agent of severe maize late wilt disease. Disease outbreak occurs at the maize flowering and fruit development stage, leading to the plugging of the plant's water vascular system, resulting in dehydration and collapse of the infected host plant. The pathogen is borne by alternative hosts, infected seeds, soil, and plant residues and gradually spreads to new areas and new countries. However, no soil assay is available today that can detect M. maydis infestation and study its prevalence. We recently developed a molecular quantitative Real-Time PCR (qPCR) method enabling the detection of the M. maydis DNA in plant tissues. Despite the technique's high sensitivity, the direct examination of soil samples can be inconsistent. To face this challenge, the current work demonstrates the use of a soil bioassay involving the cultivation of a hyper-susceptible maize genotype (Megaton cultivar, Hazera Seeds Ltd., Berurim MP Shikmim, Israel) on inspected soils. The use of Megaton cv. may facilitate pathogen establishment and spread inside the plant's tissues, and ease the isolation and enrichment of the pathogen from the soil. Indeed, this cultivar suffers from severe dehydration sudden death when grown in...Continue Reading

References

Oct 10, 1980·Nucleic Acids Research·M G Murray, W F Thompson
Sep 10, 1998·The American Surgeon·A J HerlineW C Chapman
Oct 24, 2008·Phytopathology·Amgad A SalehJohn F Leslie
Nov 10, 2011·Phytopathology·Guillaume J BilodeauFrank N Martin
Dec 11, 2014·Studies in Mycology·S KlaubaufM-H Lebrun
Feb 5, 2015·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Ofir DeganiYuval Goldblat

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Methods Mentioned

BETA
PCR

Software Mentioned

JMP
SAS

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