Soil microbiota influences clubroot disease by modulating Plasmodiophora brassicae and Brassica napus transcriptomes

Microbial Biotechnology
Stephanie DavalChristophe Mougel

Abstract

The contribution of surrounding plant microbiota to disease development has led to the 'pathobiome' concept, which represents the interaction between the pathogen, the host plant and the associated biotic microbial community, resulting or not in plant disease. The aim herein is to understand how the soil microbial environment may influence the functions of a pathogen and its pathogenesis, and the molecular response of the plant to the infection, with a dual-RNAseq transcriptomics approach. We address this question using Brassica napus and Plasmodiophora brassicae, the pathogen responsible for clubroot. A time-course experiment was conducted to study interactions between P. brassicae, two B. napus genotypes and three soils harbouring high, medium or low microbiota diversities and levels of richness. The soil microbial diversity levels had an impact on disease development (symptom levels and pathogen quantity). The P. brassicae and B. napus transcriptional patterns were modulated by these microbial diversities, these modulations being dependent on the host genotype plant and the kinetic time. The functional analysis of gene expressions allowed the identification of pathogen and plant host functions potentially involved in the cha...Continue Reading

References

Apr 25, 2001·The Plant Journal : for Cell and Molecular Biology·A SchaefferH Schaller
Nov 6, 2001·Cellular and Molecular Life Sciences : CMLS·A M Showalter
Apr 24, 2002·The Plant Cell·Klaas VandepoeleDirk Inzé
Nov 28, 2002·International Journal of Medical Microbiology : IJMM·Michel MonodOlivier Jousson
Mar 31, 2004·Cellular and Molecular Life Sciences : CMLS·R H H van den HeuvelA Mattevi
Dec 22, 2005·Infection and Immunity·Lena J HeungMaurizio Del Poeta
May 6, 2006·Molecular Plant-microbe Interactions : MPMI·Johannes SiemensJutta Ludwig-Müller
May 10, 2011·Science·Rodrigo MendesJos M Raaijmakers
Feb 14, 2012·Plant Science : an International Journal of Experimental Plant Biology·Agnieszka Kiełbowicz-Matuk
Mar 22, 2012·Plant & Cell Physiology·Antoine GravotMaria J Manzanares-Dauleux
May 9, 2012·Trends in Plant Science·Roeland L BerendsenPeter A H M Bakker
Aug 4, 2012·Nature·Derek S LundbergJeffery L Dangl
Aug 15, 2012·Nature Reviews. Microbiology·Alexander J WestermannJörg Vogel
Nov 30, 2012·Nucleic Acids Research·Christian QuastFrank Oliver Glöckner
Jan 26, 2013·The New Phytologist·Dayakar V BadriJorge M Vivanco
Jun 29, 2013·Genome Biology·Thomas R TurnerPhilip S Poole
Nov 8, 2013·The ISME Journal·Jacqueline M ChaparroJorge M Vivanco
Dec 19, 2013·PLoS Pathogens·Anja Kombrink, Bart P H J Thomma
Mar 5, 2014·Frontiers in Plant Science·Vincent Duplan, Susana Rivas
Mar 19, 2014·Frontiers in Cellular and Infection Microbiology·Muriel Vayssier-TaussatThierry Candresse
Aug 19, 2014·Trends in Plant Science·Mieke Van Lijsebettens, Klaus D Grasser
Aug 20, 2014·Molecular Plant Pathology·Jutta Ludwig-MüllerSimon Bulman
Oct 2, 2014·The Journal of Clinical Investigation·Martin J Blaser

❮ Previous
Next ❯

Citations

Mar 7, 2021·Pathogens·Arne Schwelm, Jutta Ludwig-Müller
Jul 27, 2021·Frontiers in Microbiology·Kévin GazengelStéphanie Daval
Sep 18, 2021·Plant, Cell & Environment·Xiaoli LiuDousheng Wu

❮ Previous
Next ❯

Datasets Mentioned

BETA
PRJEB36457
ERR3842608
ERR3842625
PRJEB36458
ERR3850126
ERR3850197

Methods Mentioned

BETA
RNAseq
oilseed rape
PCR

Software Mentioned

PrinSeq
blast2go
featureCounts
VennDiagram
STAR
RVAideMemoire
GnS
R
Geneclean
heatmap3

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.