Sol-gel-derived thick-film amperometric immunosensors

Analytical Chemistry
J WangK R Rogers

Abstract

Sol-gel processing is used for the first time for the preparation of electrochemical immunosensors. One-step sensor fabrication, based on the coupling of sol-gel and screen-printing technologies, is employed. A low-temperature cured ink is prepared by dispersion of rabbit immunoglobulin G (RIgG), graphite powder, and a binder in the sol-gel solution. The enzyme-labeled antibody can readily diffuse toward the encapsulated antigen, which retains its binding properties, and the association reaction is easily detected at the dispersed graphite surface. Use of anti-RIgG labeled with alkaline phosphatase, naphthyl phosphate as the substrate, and amperometric detection at +400 mV (vs Ag/AgCl) results in a low detection limit of 5 ng/mL (32 pM) for the solution antigen. Tailoring the porosity of the ceramic-carbon matrix can be used for tuning the assay performance. The high sensitivity, low cost, durability, and simplicity of the new single-use immunosensors make them well suited for various on-site applications.

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Citations

Apr 26, 2001·Biotechnology and Bioengineering·Y D KimD S Clark
Jan 6, 2007·Analytical and Bioanalytical Chemistry·Yingying SunChangqing Sun
Apr 25, 2007·Analytical and Bioanalytical Chemistry·Madalina Tudorache, Camelia Bala
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Mar 9, 2017·Journal of Neural Engineering·Alejandro Carnicer-LombarteAnne Vanhoestenberghe
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Jun 29, 1999·Analytical Chemistry·J Wang
Jun 19, 2002·Analytical Chemistry·Tzyh-Chyang TangHsuan-Jung Huang

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