Sol-gel immobilization of lactate oxidase from organic solvent: toward the advanced lactate biosensor

Analytical Chemistry
Eugenia I YashinaArkady A Karyakin

Abstract

We report on the novel protocol for enzyme immobilization into gel of siloxanes using water-organic mixtures with the high content of organic solvent as a reaction medium. Hydrolysis of alkoxysilanes carried out without excessive dilution with water resulted in more active and stable enzyme containing membranes. Immobilization of an inherently labile lactate oxidase according to the proposed sol-gel protocol over Prussian Blue modified electrode resulted in an advanced lactate biosensor characterized with a sensitivity of 0.18 A M(-1) cm(-2) in the flow injection analysis (FIA) mode over a wide dynamic range. A comparison with the known sensors has shown that analytical performances of the elaborated lactate biosensor are advantageous over both published systems and commercialized devices. The biosensor shows an appropriate stability and is suitable for clinical analysis (including noninvasive diagnostics) and food quality control.

References

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Citations

Nov 3, 2011·Analytical Chemistry·Danielle W KimmelDavid E Cliffel
Sep 17, 2013·Analytical and Bioanalytical Chemistry·Liza RassaeiAlbert van den Berg
Sep 27, 2014·Bulletin of Experimental Biology and Medicine·A L RusanovM Yu Shkurnikov
Aug 29, 2014·Analytical Chemistry·Alina N SekretaryovaMikhail Yu Vagin
Jun 22, 2014·Trends in Microbiology·Tianyi JiangPing Xu
Jan 7, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Nicholas G PoulosMichael C Leopold
Oct 23, 2013·Biosensors & Bioelectronics·Alina N SekretaryovaArkady A Karyakin
May 26, 2017·Analytical Chemistry·Maria A KomkovaArkady A Karyakin
Jan 1, 2012·Membranes·Andrew KavanaghKevin J Fraser
Aug 15, 2018·Sensors·Gerard CumminsMarc P Y Desmulliez
Dec 11, 2019·Talanta·Darya V VokhmyaninaArkady A Karyakin
Oct 27, 2017·Analytical Chemistry·Nikolay V ZaryanovArkady A Karyakin

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