Solanine Inhibits Immune Escape Mediated by Hepatoma Treg Cells via the TGFβ/Smad Signaling Pathway.

BioMed Research International
Juwei GaoYiyi Cui

Abstract

To observe the inhibitory effect of solanine on regulatory T cells (Treg) in transplanted hepatoma mice and to study the mechanism of solanine inhibiting tumor growth. The levels of Treg cells and IL-2, IL-10, and TGFβ in the blood of patients with liver cancer were detected by flow cytometry and ELISA, respectively. A mouse hepatocellular carcinoma (HCC) graft model was established and randomly divided into four groups: control group, solanine group, TGFβ inhibitor group (SB-431542), and solanine +TGFβ inhibitor combined group. Tumor volume of each group was recorded, tumor inhibition rate was calculated, and tumor metastasis was counted. The proportion of CD4+CD25+Foxp3+ Treg in transplanted tumor tissues was detected by flow cytometry. The expression levels of Foxp3 and TGFβ in transplanted tumor tissues were detected by quantitative fluorescence PCR. Compared with healthy people, Treg cells and IL-2, IL-10, and TGFβ contents in peripheral blood of liver cancer patients were increased. The results of the transplanted tumor model in mice showed that the tumor volume of the transplanted mice in the solanine group and the TGFβ inhibitor mice was reduced compared with the control group. The combined group had the smallest tumor ...Continue Reading

References

Dec 13, 2002·Vaccine·Michael CampoliSoldano Ferrone
Mar 27, 2003·Nature Reviews. Immunology·Jeffrey A Bluestone, Abul K Abbas
Apr 22, 2003·Journal of Cellular Physiology·Angel Garcia-LoraFederico Garrido
May 19, 2004·The Journal of Experimental Medicine·Irina Apostolou, Harald von Boehmer
Dec 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maria A Curotto de LafailleJuan J Lafaille
Dec 30, 2004·Proceedings of the National Academy of Sciences of the United States of America·Mei-Ling ChenKhashayarsha Khazaie
Aug 19, 2007·Medicinal Research Reviews·Simone Mocellin, Donato Nitti
Apr 9, 2008·European Journal of Immunology·David A HorwitzJ Dixon Gray
Nov 5, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anya SchneiderJane H Buckner
Nov 28, 2009·European Journal of Immunology·Ling LuSong Guo Zheng
Feb 9, 2011·Immunology and Cell Biology·Makoto Miyara, Shimon Sakaguchi
Nov 5, 2011·The Journal of Allergy and Clinical Immunology·Oscar PalomaresCezmi A Akdis
May 3, 2012·Cancer Research·Andrea FacciabeneGeorge Coukos
Jul 6, 2013·Archives of Gynecology and Obstetrics·Jörg B EngelSabine E Segerer
Sep 21, 2013·European Journal of Pharmacology·Maryam MohsenikiaMohammad Ali Mohagheghi
Apr 11, 2018·Immunology and Cell Biology·Salman M Toor, Eyad Elkord
Jul 4, 2019·Journal of Experimental & Clinical Cancer Research : CR·Weilong ZhongCheng Yang

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Methods Mentioned

BETA
ELISA
enzyme-linked immunosorbent assay
Transfection
flow cytometry

Software Mentioned

SPSS

Related Concepts

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