Solexa sequencing identification of conserved and novel microRNAs in backfat of Large White and Chinese Meishan pigs.

PloS One
Chen ChenSiwen Jiang

Abstract

The domestic pig (Sus scrofa), an important species in animal production industry, is a right model for studying adipogenesis and fat deposition. In order to expand the repertoire of porcine miRNAs and further explore potential regulatory miRNAs which have influence on adipogenesis, high-throughput Solexa sequencing approach was adopted to identify miRNAs in backfat of Large White (lean type pig) and Meishan pigs (Chinese indigenous fatty pig). We identified 215 unique miRNAs comprising 75 known pre-miRNAs, of which 49 miRNA*s were first identified in our study, 73 miRNAs were overlapped in both libraries, and 140 were novelly predicted miRNAs, and 215 unique miRNAs were collectively corresponding to 235 independent genomic loci. Furthermore, we analyzed the sequence variations, seed edits and phylogenetic development of the miRNAs. 17 miRNAs were widely conserved from vertebrates to invertebrates, suggesting that these miRNAs may serve as potential evolutional biomarkers. 9 conserved miRNAs with significantly differential expressions were determined. The expression of miR-215, miR-135, miR-224 and miR-146b was higher in Large White pigs, opposite to the patterns shown by miR-1a, miR-133a, miR-122, miR-204 and miR-183. Almost a...Continue Reading

References

Mar 22, 1994·Proceedings. Biological Sciences·P SchusterI L Hofacker
Aug 11, 2000·Science·S E RossO A MacDougald
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 11, 2002·The Journal of Biological Chemistry·Christina N BennettOrmond A MacDougald
May 8, 2004·Genes & Development·Marianthi KiriakidouArtemis Hatzigeorgiou
May 12, 2004·Journal of Molecular Biology·Andrea Tanzer, Peter F Stadler
Jun 24, 2004·Nature Reviews. Genetics·Lin He, Gregory J Hannon
Sep 17, 2004·Nature·Victor Ambros
Oct 27, 2004·PLoS Biology·Bino JohnDebora S Marks
Sep 13, 2005·FEBS Letters·Alexei Aravin, Thomas Tuschl
Nov 30, 2005·Nucleic Acids Research·Caifu ChenKarl J Guegler
Dec 6, 2005·Developmental Biology·Baohong ZhangTodd A Anderson
Feb 7, 2006·Cell Metabolism·Christine EsauBrett P Monia
Apr 6, 2006·Genome Biology·Matthew J BlowMichael R Stratton
Jun 1, 2006·Nature Genetics·Bryan R Cullen
Jul 18, 2006·Nucleic Acids Research·Jan Krüger, Marc Rehmsmeier
Jan 2, 2007·Trends in Cell Biology·Ramesh S PillaiWitold Filipowicz
Jan 11, 2007·Genome Génome / Conseil National De Recherches Canada·Hee-Jeong KimNam-Hyung Kim
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
May 31, 2007·Proceedings of the National Academy of Sciences of the United States of America·J Robin LytleJoan A Steitz
Feb 23, 2008·Journal of Hepatology·Muriel GirardAlexandra Henrion-Caude
Jun 13, 2008·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Jung KimYoung Sik Lee
Aug 30, 2008·Biochemical and Biophysical Research Communications·Soroush Sharbati-TehraniRalf Einspanier
Sep 13, 2008·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Christelle Borel, Stylianos E Antonarakis
Feb 12, 2009·BMC Genomics·Tara G McDaneldRalph T Wiedmann
Mar 4, 2009·Nucleic Acids Research·H Alexander EbhardtRichard P Fahlman
Mar 28, 2009·Molecular Endocrinology·Tingwan SunDavid J Mangelsdorf
Mar 31, 2009·FEBS Letters·Tina RathjenTamas Dalmay
Apr 7, 2009·The FEBS Journal·Qun LinZhong Yun
May 9, 2009·Gene·Baohong ZhangXiaoping Pan
Jan 12, 2010·Biochemical and Biophysical Research Communications·Sang Yun KimJae Bum Kim
Mar 5, 2010·BMC Genomics·Shiping LiuQingyou Xia
Oct 12, 2010·FEBS Letters·Ana Laura Gutierrez AguilarGunter Meister
Dec 22, 2010·BMC Genomics·Yijun MengMing Chen
Feb 12, 2011·Journal of Cellular Biochemistry·Guoxi LiGongshe Yang

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Citations

May 28, 2014·Acta Biochimica Et Biophysica Sinica·Peiyue DongShiduo Sun
May 23, 2014·Journal of Molecular Endocrinology·Chen ChenSiwen Jiang
Oct 17, 2013·International Journal of Molecular Sciences·Lifan ZhangNingying Xu
Jul 5, 2013·Animal : an International Journal of Animal Bioscience·X WangH Jiang
Oct 16, 2015·Molecular Genetics and Genomics : MGG·Xinhua HouKui Li
May 15, 2013·The International Journal of Biochemistry & Cell Biology·Yongdong PengSiwen Jiang
Nov 12, 2014·PloS One·Xingguo WangZhiliang Gu
Jul 30, 2015·PloS One·Caroline M Junker MentzelMerete Fredholm
Apr 15, 2018·Journal of Physiology and Biochemistry·Chen ChenYinglin Peng
Sep 7, 2019·Molecular Reproduction and Development·Yu HuXian-Zhong Wang
Mar 25, 2020·Journal of Animal Physiology and Animal Nutrition·Jiayun QiaoYupeng Li
Oct 13, 2017·Cell and Tissue Research·Lindsay R Stolzenburg, Ann Harris
Jul 21, 2016·Journal of the Science of Food and Agriculture·Yunjing LiGang Wu
Nov 7, 2015·The Biochemical Journal·Lindsay R StolzenburgAnn Harris
Mar 7, 2020·Animals : an Open Access Journal From MDPI·Klavdija PoklukarMartin Škrlep
Jun 29, 2016·Journal of Cellular Physiology·Antonio Casado-DíazGabriel Dorado

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Methods Mentioned

BETA
Solexa sequencing
PCR

Software Mentioned

RNA22
RNAfold
DIANA LAB
MEGA4
BLASTN
TargetScan
Solexa
PicTar
RNAhybrid

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