Solid lipid nanoparticles release DNA upon endosomal acidification in human embryonic kidney cells

Nanotechnology
A Radaic, Marcelo B de Jesus

Abstract

Nanotechnology can produce materials with unique features compared to their bulk counterparts, which can be useful for medical applications (i.e. nanomedicine). Among the therapeutic agents used in nanomedicine, small molecules or biomacromolecules, such as proteins or genetic materials, can be designed for disease diagnostics and treatment. To transport these biomacromolecules to the target cells, nanomedicine requires nanocarriers. Solid lipid nanoparticles (SLNs) are among the promising nanocarriers available, because they can be made from biocompatible materials and present high stability (over one year). In addition, upon the binding genetic material, SLNs form SLNplexes. However, little is yet known about how cells process these SLNplexes-in particular, how internalization and endosome acidification affects the transfection mediated by SLNplexes. Therefore, we aim to investigate how these processes affect SLNplex transfection in HEK293T cells. We find that the SLNplex is mainly internalized by clathrin-mediated endocytosis, which is a fast and reliable pathway to transfection, leading to approximately 60% transfection efficiency. Interestingly, upon acidification (below pH 5.0), the SLN seems to release its DNA content, w...Continue Reading

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Citations

Apr 23, 2020·Pharmaceutics·Darío Manzanares, Valentín Ceña
Jun 27, 2019·Drug Delivery and Translational Research·Monique Culturato Padilha MendonçaMarcelo Bispo de Jesus
Dec 24, 2018·Nanomedicine·Agostina GrilloneGianni Ciofani
Dec 4, 2020·International Journal of Nanomedicine·Xiaowei WangRongguang Shao
Jan 19, 2021·Expert Opinion on Drug Delivery·Mohammad HajimolaaliFarid Dorkoosh
May 23, 2020·Nanomedicine : Nanotechnology, Biology, and Medicine·Allan Radaic, Daniel Martins-de-Souza
Mar 30, 2021·Computational and Structural Biotechnology Journal·Allan Radaic, Yvonne L Kapila

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