PMID: 7008176Jan 1, 1980Paper

Solid phase ELISA for serum ferritin

Scandinavian Journal of Clinical and Laboratory Investigation
M PagéM Nilsson

Abstract

We describe a solid-phase enzyme immunoassay for human serum ferritin, with the use of polyethylene glycol to shorten the reaction. The amount of bound enzyme-antibody conjugate used as a second site antibody is proportional to the ferritin titre in the assay. This method is fast, and the reagents are stable for many months at 4 degrees C. The lowest detectable concentration was 5 micrograms/l. Interassay coefficients of variation were 9.3 and 12.2%, respectively, at ferritin concentrations of 100 micrograms/l and 200 micrograms/l. The data also show that polyethylene glycol accelerates antigen-antibody complex formation in the solid phase. An assay may be completed in 5 h.

References

Aug 1, 1977·Scandinavian Journal of Haematology·M A SiimesP R Dallman
Sep 1, 1976·British Journal of Cancer·A JacobsD Y Wang
Aug 22, 1975·Annals of the New York Academy of Sciences·Y NiitsuI Urushizaki
Feb 8, 1975·Clinica Chimica Acta; International Journal of Clinical Chemistry·J W HallidayL W Powell
Oct 1, 1975·Journal of the National Cancer Institute·D M Marcus, N Zinberg
Sep 1, 1975·British Journal of Haematology·A Jacobs, M Worwood
Sep 1, 1974·Analytical Biochemistry·L E MilesJ D Cook

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