PMID: 8946666Dec 1, 1996Paper

Solid-phase extraction and high-performance liquid chromatographic analysis of clozapine and norclozapine in human plasma

Therapeutic Drug Monitoring
D J FreemanK Oyewumi

Abstract

A rapid high-performance liquid chromatographic method for the simultaneous quantitation of plasma clozapine and its principal metabolite, norclozapine, was developed using a Spherisorb C8 5-micron reverse-phase column and an acetonitrile/potassium phosphate buffer/triethylamine mobile phase. Clozapine and norclozapine were extracted from plasma on solid-phase reusable microcolumns. Absolute recovery of clozapine, norclozapine, and the internal standard, flurazepam, was quantitative. The mean intraassay coefficient of variation was better than 6% for clozapine and norclozapine at therapeutic concentrations. Based on a plasma sample volume of 200 microliters, the limit of accurate quantitation of both clozapine and norclozapine was approximately 25 micrograms/L (coefficients of variation = 11.4% and 8.4%, respectively). The method has been used over the past 2 years for single-dose pharmacokinetic studies and for monitoring patients receiving clozapine therapy.

References

Jan 1, 1988·European Journal of Clinical Pharmacology·Y F ChengJ Gabrielsson
Apr 1, 1995·Therapeutic Drug Monitoring·L K OyewumiD Vollick
Feb 1, 1993·Clinical Pharmacokinetics·M W JannW H Chang

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Citations

Apr 12, 2000·British Journal of Clinical Pharmacology·P B Mitchell
May 4, 2002·Canadian Journal of Psychiatry. Revue Canadienne De Psychiatrie·L Kola OyewumiDavid L Streiner
Apr 8, 2009·Therapeutic Drug Monitoring·Wenzie NgRobert R Bies
Nov 15, 2012·Therapeutic Drug Monitoring·Lisbeth PatteetHugo Neels
Jan 7, 2000·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·B Buur-Rasmussen, K Brøsen

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