Solid-phase extraction as sample preparation of water samples for cell-based and other in vitro bioassays

Environmental Science. Processes & Impacts
Peta A NealeBeate I Escher

Abstract

In vitro bioassays are increasingly used for water quality monitoring. Surface water samples often need to be enriched to observe an effect and solid-phase extraction (SPE) is commonly applied for this purpose. The applied methods are typically optimised for the recovery of target chemicals and not for effect recovery for bioassays. A review of the few studies that have evaluated SPE recovery for bioassays showed a lack of experimentally determined recoveries. Therefore, we systematically measured effect recovery of a mixture of 579 organic chemicals covering a wide range of physicochemical properties that were spiked into a pristine water sample and extracted using large volume solid-phase extraction (LVSPE). Assays indicative of activation of xenobiotic metabolism, hormone receptor-mediated effects and adaptive stress responses were applied, with non-specific effects determined through cytotoxicity measurements. Overall, effect recovery was found to be similar to chemical recovery for the majority of bioassays and LVSPE blanks had no effect. Multi-layer SPE exhibited greater recovery of spiked chemicals compared to LVSPE, but the blanks triggered cytotoxicity at high enrichment. Chemical recovery data together with single che...Continue Reading

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Citations

May 31, 2018·Environmental Toxicology and Chemistry·Beate I EscherDaniel L Villeneuve
Sep 14, 2018·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Jian LiNengsheng Ye
Aug 26, 2018·Integrated Environmental Assessment and Management·Milou Ml DingemansAnnemarie P van Wezel
Mar 11, 2020·Environmental Science and Pollution Research International·Adebayo I Farounbi, Nosiphiwe P Ngqwala
Jun 26, 2020·Frontiers in Chemistry·Gustavo Bodelón, Isabel Pastoriza-Santos
Jan 25, 2020·Science·Beate I EscherEmma L Schymanski
Feb 25, 2021·Journal of Toxicology and Environmental Health. Part a·Elke DoppJochen Tuerk
Jul 18, 2019·Chemical Research in Toxicology·Beate I EscherRita Schlichting
May 24, 2019·Environmental Science & Technology·Johannes VölkerMartin Wagner
May 14, 2020·Environmental Science & Technology·Wendi FangXiaowei Zhang
Dec 23, 2021·Analytical and Bioanalytical Chemistry·Christoph D RummelMechthild Schmitt-Jansen

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