Solid-phase extraction in combination with dispersive liquid-liquid microextraction and ultra-high performance liquid chromatography-tandem mass spectrometry analysis: the ultra-trace determination of 10 antibiotics in water samples

Analytical and Bioanalytical Chemistry
Ning LiangLongshan Zhao

Abstract

A novel method, solid-phase extraction combined with dispersive liquid-liquid microextraction (SPE-DLLME), was developed for ultra-preconcentration of 10 antibiotics in different environmental water samples prior to ultra-high performance liquid chromatography-tandem mass spectrometry detection. The optimized results were obtained as follows: after being adjusted to pH 4.0, the water sample was firstly passed through PEP-2 column at 10 mL min(-1), and then methanol was used to elute the target analytes for the following steps. Dichloromethane was selected as extraction solvent, and methanol/acetonitrile (1:1, v/v) as dispersive solvent. Under optimal conditions, the calibration curves were linear in the range of 1-1000 ng mL(-1) (sulfamethoxazole, cefuroxime axetil), 5-1000 ng mL(-1) (tinidazole), 10-1000 ng mL(-1) (chloramphenicol), 2-1000 ng mL(-1) (levofloxacin oxytetracycline, doxycycline, tetracycline, and ciprofloxacin) and 1-400 ng mL(-1) (sulfadiazine) with a good precision. The LOD and LOQ of the method were at very low levels, below 1.67 and 5.57 ng mL(-1), respectively. The relative recoveries of the target analytes were in the range from 64.16% to 99.80% with relative standard deviations between 0.7 and 8.4%. The ma...Continue Reading

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Citations

Feb 12, 2020·Journal of Separation Science·Xintong XieGangfeng Ouyang
Aug 28, 2017·Environmental Science and Pollution Research International·Martina IvešićNives Galić
May 21, 2020·Royal Society Open Science·Salwani Md SaadNoorfatimah Yahaya
May 13, 2017·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Xiaoxia Li, Kyung Ho Row
Oct 28, 2019·Critical Reviews in Analytical Chemistry·Xiao-Yi DuanXue-Song Feng
Apr 23, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yuanchen LiuYusuke Yamauchi
Dec 18, 2021·Journal of Separation Science·Guizhen Li, Kyung Ho Row

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