Solid-phase extraction of arginine vasotocin and isotocin in fish samples and subsequent gradient reversed-phase high-performance liquid chromatographic separation

Journal of Chromatography. B, Biomedical Applications
Ewa Kulczykowska

Abstract

Gradient high-performance liquid chromatography (HPLC) preceded by a solid-phase extraction (SPE) step is described for determining arginine vasotocin and isotocin, the neurohypophysial nonapeptides, in fish plasma samples. The combination of these two methods significantly improves the separation and increases the sensitivity of the assay. The proposed assay may be a useful alternative for analysis of similar nonapeptides in plasma without the use of radioisotopes, while taking into consideration a difference in detection sensitivity.

References


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Citations

Nov 13, 2012·Fish Physiology and Biochemistry·Magdalena GozdowskaEwa Kulczykowska
Jun 4, 2014·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Hanna Kalamarz-KubiakHanna Rosenfeld
Nov 26, 2010·General and Comparative Endocrinology·Arnau Rodríguez-IllamolaJesús M Míguez
Mar 31, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Diego Sebastián Insuasty CepedaZuly Jenny Rivera Monroy

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