Solid-phase extraction with end-capped C2 columns for the routine measurement of racemic citalopram and metabolites in plasma by high-performance liquid chromatography

Journal of Chromatography. B, Biomedical Sciences and Applications
B Carlsson, B Norlander

Abstract

An assay based on solid-phase extraction followed by high-performance liquid chromatography (HPLC) was developed for the measurement of citalopram and its main metabolites desmethylcitalopram and didesmethylcitalopram. The best extraction procedure was performed with end-capped C2 column utilising secondary silanol interactions to obtain clean extract. The HPLC analysis was done on a phenyl column with a mobile phase without any amine additives. Fluorescence detection gave a limit of detection of 0.8 nmol/l plasma for the compounds analysed.

Citations

Nov 4, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Sonu Sundd SinghRuchy Shah
Oct 6, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·G TournelM Lhermitte
Aug 13, 2002·Journal of Clinical Psychopharmacology·Margareta ReisFinn Bengtsson
Dec 31, 2010·Journal of Clinical Psychopharmacology·Maria D ChermáPer A Gustafsson
Jan 22, 2002·Therapeutic Drug Monitoring·B CarlssonJ Ahlner
Feb 2, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Tao JiangHongzhuan Chen

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