Solid phase protein chemical synthesis

Topics in Current Chemistry
Laurent RaibautOleg Melnyk

Abstract

The chemical synthesis of peptides or small proteins is often an important step in many research projects and has stimulated the development of numerous chemical methodologies. The aim of this review is to give a substantial overview of the solid phase methods developed for the production or purification of polypeptides. The solid phase peptide synthesis (SPPS) technique has facilitated considerably the access to short peptides (<50 amino acids). However, its limitations for producing large homogeneous peptides have stimulated the development of solid phase covalent or non-covalent capture purification methods. The power of the native chemical ligation (NCL) reaction for protein synthesis in aqueous solution has also been adapted to the solid phase by the combination of novel linker technologies, cysteine protection strategies and thioester or N,S-acyl shift thioester surrogate chemistries. This review details pioneering studies and the most recent publications related to the solid phase chemical synthesis of large peptides and proteins.

Citations

Aug 18, 2016·Journal of the American Chemical Society·Michael T JacobsenMichael S Kay
Dec 5, 2016·Journal of Biotechnology·Oksana NekrasovaAlexey Feofanov
Oct 12, 2018·The Journal of Biological Chemistry·Marianne Paolini-BertrandOliver Hartley
Feb 6, 2018·Experimental and Therapeutic Medicine·Yun-Tian Yang, Shan Jin
Sep 13, 2016·Chembiochem : a European Journal of Chemical Biology·Shugo TsudaTaku Yoshiya
Jan 14, 2017·Frontiers in Cellular and Infection Microbiology·Margit MahlapuuCamilla Björn
Apr 27, 2016·Journal of Peptide Science : an Official Publication of the European Peptide Society·Martin Engelhard
May 26, 2017·Nature Communications·Helen M BurkeEoin M Scanlan
Mar 30, 2019·Polymer Chemistry·Deborah A BarkleyJonathan G Rudick
Jul 5, 2017·Chemical & Pharmaceutical Bulletin·Masayori HagimoriShigeru Kawakami
Apr 4, 2020·Current Opinion in Chemical Biology·Vangelis AgouridasOleg Melnyk
Nov 9, 2019·Bioconjugate Chemistry·Jennifer BouchennaOleg Melnyk
Aug 19, 2021·Macromolecular Bioscience·Fengze MiaoQuangang Zhu
Sep 1, 2021·Peptides·Ernesto M MartellAnselmo J Otero-González

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