PMID: 7031131Jan 1, 1981Paper

Solid-phase selection of human T lymphocyte subpopulations using monoclonal antibodies

Journal of Immunological Methods
S FongJ H Vaughan

Abstract

This report describes a novel solid-phase technique for the positive selection of human T lymphocyte subsets labeled by indirect immunofluorescence with monoclonal anti-bodies. Fluorescein labeled normal human T cells or a T cell line were fractionated on plastic culture dishes precoated with affinity chromatography purified anti-fluorescein antibodies. Cell binding was specific for fluorescein, and was both time and temperature dependent. Bound cells were eluted at 37 degrees C with fluorescein-L-lysine. The eluted cells were enriched with highly viable and functional human T cell subsets. Thus Leu3 monoclonal antibody selected cells were shown to provide helper activity in the pokeweed mitogen induced IgM and IgG immunoglobulin secretory response of autologous B cells. The Leu2 antibody selected T cells suppressed both IgM and IgG secretory responses. In addition, studies with the monoclonal antibody 1G11, which binds to an antigen expressed on acute lymphocytic leukemia (T-ALL) cells and the T-ALL derived cell line RPMI-8402, demonstrated that this solid-phase technique can be used to select for cells which are present at low frequencies in a mixed population. It thus provides a simple and reproducible means for the preparat...Continue Reading

References

Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E L ReinherzS F Schlossman
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R LevyR Warnke
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·L J Wysocki, V L Sato
Jun 11, 1968·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·E A BoyseL J Old
Mar 1, 1974·The Journal of Experimental Medicine·T K ChoiA Nisonoff
Jan 1, 1968·Scandinavian Journal of Clinical and Laboratory Investigation·C E Mogensen
Jan 1, 1981·Journal of Immunological Methods·S FongJ H Vaughan
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R L EvansR A Good
Jan 1, 1964·The International Journal of Social Psychiatry·E C TRAUTMAN

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Citations

Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R AuerbachS L Watt
Jun 1, 1983·The Journal of Experimental Medicine·M J CaulfieldJ Cerny
Jan 1, 1982·Immunological Communications·J M HuntB A Laishes
Sep 1, 1988·Journal of Clinical Immunology·M R LieblingJ S Louie

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