PMID: 7544461Jul 25, 1995Paper

Solid phase synthesis of 5'-diphosphorylated oligoribonucleotides and their conversion to capped m7Gppp-oligoribonucleotides for use as primers for influenza A virus RNA polymerase in vitro

Nucleic Acids Research
G G BrownleeJ J Dalluge

Abstract

We have synthesized four different 5'-diphosphorylated oligoribonucleotides, varying in length from 11 to 13 nucleotides by a new solid phase method. After deprotection and partial purification the 5'-diphosphorylated oligoribonucleotides could be converted to capped (m7Gppp) oligoribonucleotides using guanylyl transferase. Radiolabelled capped oligoribonucleotides acted as primers for the influenza A virus RNA polymerase in vitro. The solid phase method described here should also allow the addition of 5'-diphosphates to synthetic oligodeoxyribonucleotides and be capable automation.

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Citations

Jul 4, 2007·Molecular and Cellular Biology·Jesse R ZamudioDavid A Campbell
Jun 9, 2009·Molecular Microbiology·Jesse R ZamudioNancy R Sturm
Jul 19, 2011·Chemistry : a European Journal·Viktoria Caroline Tonn, Chris Meier
Jun 9, 2016·Nature Microbiology·Aartjan J W Te VelthuisErvin Fodor
Dec 29, 2004·Nucleosides, Nucleotides & Nucleic Acids·I I Koukhareva, A V Lebedev
Sep 21, 2001·Nucleosides, Nucleotides & Nucleic Acids·A V LebedevM M Vaghefi
Sep 8, 2012·Current Protocols in Nucleic Acid Chemistry·Ivan ZlatevFrançois Morvan
Apr 16, 2005·The Journal of General Virology·Pierre Fechter, George G Brownlee
Mar 21, 2003·The Journal of Biological Chemistry·Pierre FechterGeorge G Brownlee
Feb 29, 2000·The Journal of Biological Chemistry·K KlumppB Handa
Aug 31, 2016·Nature Microbiology·Aartjan J W Te VelthuisErvin Fodor
Nov 10, 2009·The Journal of Organic Chemistry·Céline CrausteSuzanne Peyrottes

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