Solid-phase synthesis of protein-polymers on reversible immobilization supports

Nature Communications
Hironobu MurataAlan J Russell

Abstract

Facile automated biomacromolecule synthesis is at the heart of blending synthetic and biologic worlds. Full access to abiotic/biotic synthetic diversity first occurred when chemistry was developed to grow nucleic acids and peptides from reversibly immobilized precursors. Protein-polymer conjugates, however, have always been synthesized in solution in multi-step, multi-day processes that couple innovative chemistry with challenging purification. Here we report the generation of protein-polymer hybrids synthesized by protein-ATRP on reversible immobilization supports (PARIS). We utilized modified agarose beads to covalently and reversibly couple to proteins in amino-specific reactions. We then modified reversibly immobilized proteins with protein-reactive ATRP initiators and, after ATRP, we released and analyzed the protein polymers. The activity and stability of PARIS-synthesized and solution-synthesized conjugates demonstrated that PARIS was an effective, rapid, and simple method to generate protein-polymer conjugates. Automation of PARIS significantly reduced synthesis/purification timelines, thereby opening a path to changing how to generate protein-polymer conjugates.

References

Nov 1, 1991·Enzyme and Microbial Technology·R BahulekarS Ponrathnam
Aug 1, 1992·Protein Expression and Purification·J Porath
May 10, 2000·Trends in Biotechnology·H GrienglM Fechter
Feb 24, 2001·Nature·A M Klibanov
Dec 12, 2002·Chemical Reviews·Lizbeth Hedstrom
Mar 4, 2003·Nature Reviews. Drug Discovery·J Milton Harris, Robert B Chess
May 17, 2003·Nature Reviews. Drug Discovery·Ruth Duncan
Jan 1, 1988·Biotechnology Advances·O P Ward, M Moo-Young
Jul 28, 2005·Journal of the American Chemical Society·Britto S SandanarajS Thayumanavan
Nov 15, 2005·Biomacromolecules·Bhalchandra S LeleAlan J Russell
Dec 1, 2005·Journal of the American Chemical Society·Karina L HerediaHeather D Maynard
Oct 13, 2006·Current Opinion in Chemical Biology·Yanjie Zhang, Paul S Cremer
Oct 6, 2009·Biochimica Et Biophysica Acta·Hana SanderováLibor Krásný
Apr 18, 2012·Chemical Reviews·Awanish Kumar, Pannuru Venkatesu
Jul 16, 2013·Biomaterials·Chad CummingsAlan J Russell
Sep 13, 2014·Journal of the American Chemical Society·Emma M Pelegri-O'DayHeather D Maynard
Nov 18, 2014·Nature Materials·Isidro CoboSébastien Perrier
Jan 24, 2015·Bioconjugate Chemistry·Yuhui GongMarc A Gauthier
Mar 18, 2015·Biotechnology Advances·Oveimar BarbosaRoberto Fernandez-Lafuente
Jan 15, 2016·Biomacromolecules·Sergey A IsarovJonathan K Pokorski
Jan 14, 2017·Biomacromolecules·Chad S CummingsAlan J Russell

❮ Previous
Next ❯

Citations

Oct 18, 2018·Angewandte Chemie·Alan E EncisoKrzysztof Matyjaszewski
Nov 26, 2020·International Journal of Molecular Sciences·Akanksha TyagiDeog-Hwan Oh
Nov 19, 2019·Biomacromolecules·Stefanie L BakerKrzysztof Matyjaszewski
May 25, 2021·Frontiers in Molecular Biosciences·Maria Luisa Di VonaMarco Sette
Jul 25, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Lara R Lechlitner, Onofrio Annunziata

❮ Previous
Next ❯

Methods Mentioned

BETA
infrared spectroscopy
acylation
protein assay
PARIS-CT
dynamic light scattering
protein
nuclear magnetic resonance
NMR

Software Mentioned

Enzfitter
PARIS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Midwifery Today and Childbirth Education
S G Jonquil
Biopolymers
Anna Maria PapiniCo-editors and PIPS organizers
Organic & Biomolecular Chemistry
Karina L Heredia, Heather D Maynard
The Journal of the Albert Einstein Medical Center, Philadelphia
S WINSTEN
© 2022 Meta ULC. All rights reserved