Jan 1, 1996

Solid phase technique versus gel centrifugation for detection of erythrocyte antibodies--a prospective study comparison the 2 antibody detection tests

Beiträge zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine
T ZeilerV Kretschmer


To define differences of specifity and sensitivity between a solid phase- and a gel centrifugation test for detection of irregular erythrocyte antibodies. 3052 blood samples were screened for erythrocyte antibodies (AB) by gel centrifugation [ID-System, (ID) 37 degrees C Bromelin and indirect antiglobulin test with LISS (IAT)] and a solid phase antiglobulin test [Capture-R Ready Screen (CR)] in a prospective study. Identical test cells were used as immobilized monolayer in CR and as 1%-suspension in ID. Additionally, 42 sera with antibodies reacting in both tests were titered geometrically in both techniques. In 79 (2.6%) of all sera tested, irregular erythrocyte antibodies were detected. 64 (81%) of these positive sera were detected by both tests, 73 (92.3%) by ID and 70 (88.4%) by CR. 6 sera with AB (7.6%) were positive only with CR (1 Ce, 1 E, 1 Leb, 1 C, 1 D, 1 Cob) and 9 (11.4%) only with ID (4 Lea, 1 Fya, 1 C, 2 P1, 1 D). Seven AB solely detected in ID only reacted in the bromelin test, besides an anti-Fya, which was an IgM antibody. The titration of IgG antibodies showed a slightly higher sensitivity of the CR (less than one titre step). The ID showed clearly more unspecific reactions (1.1%) than the CR (0.5%). The antib...Continue Reading

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Mentioned in this Paper

Blood Grouping and Crossmatching
Coombs Test
Procedure to Identify Antibody
Antibody Screen
Phosphoglycerate Dehydrogenase Activity
Indirect Coombs Test
IgM Immunoglobulin Complex, Circulating

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