Solid-state, dye-labeled DNA detects volatile compounds in the vapor phase.

PLoS Biology
Joel WhiteJ S Kauer

Abstract

This paper demonstrates a previously unreported property of deoxyribonucleic acid-the ability of dye-labeled, solid-state DNA dried onto a surface to detect odors delivered in the vapor phase by changes in fluorescence. This property is useful for engineering systems to detect volatiles and provides a way for artificial sensors to emulate the way cross-reactive olfactory receptors respond to and encode single odorous compounds and mixtures. Recent studies show that the vertebrate olfactory receptor repertoire arises from an unusually large gene family and that the receptor types that have been tested so far show variable breadths of response. In designing biomimetic artificial noses, the challenge has been to generate a similarly large sensor repertoire that can be manufactured with exact chemical precision and reproducibility and that has the requisite combinatorial complexity to detect odors in the real world. Here we describe an approach for generating and screening large, diverse libraries of defined sensors using single-stranded, fluorescent dye-labeled DNA that has been dried onto a substrate and pulsed with brief exposures to different odors. These new solid-state DNA-based sensors are sensitive and show differential, se...Continue Reading

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Jan 25, 2008·PLoS Biology·Joel WhiteJohn S Kauer

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Citations

Apr 27, 2010·PLoS Computational Biology·Rafi HaddadNoam Sobel
Sep 8, 2015·Sensors·Souhir BoujdayWolfgang Knoll
Jan 25, 2008·PLoS Biology·Joel WhiteJohn S Kauer
Aug 20, 2010·Angewandte Chemie·Florent SamainEric T Kool
Mar 29, 2019·PloS One·Kimberly A KoesterKaterina A Christopoulos
Sep 1, 2008·Journal of Breath Research·A Gelperin, A T C Johnson
Jan 4, 2012·Macromolecular Rapid Communications·Marius ChyasnavichyusIgor Luzinov
Dec 10, 2016·Sensors and Actuators. B, Chemical·Nalin KattaBaranidharan Raman
May 24, 2012·Chemical Reviews·Marc VendrellYoung-Tae Chang
Oct 12, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Kan FuBrian G Willis
Nov 15, 2011·ACS Chemical Neuroscience·Baranidharan RamanSteve Semancik
May 16, 2020·ACS Sensors·Christine K McGinnIoannis Kymissis

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Methods Mentioned

BETA
Fluorescence
PCR

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