Solid-State Nanopore Single-Molecule Sensing of DNAzyme Cleavage Reaction Assisted with Nucleic Acid Nanostructure

ACS Applied Materials & Interfaces
Libo ZhuQuanjun Liu

Abstract

The detection and investigation of biomolecules at a single-molecule level is important for improving diagnosis in biomedicine. Solid-state nanopores are a unique tool that have the potential to accomplish this task because they are label-free and require only low sample consumption. However, the event-readouts of current small polymer molecules are still limited because of its relatively large size and low signal-to-noise ratios. Here, we present a rapid sensing approach for the detection of GR-5 DNAzyme cleaving specific substrate reactions using relatively larger size silicon nitride nanopores by introducing a type of nucleic acid nanostructure (DNA tetrahedron) as a carrier. The proposed method is convenient and sensitive enough to detect the cleavage reactions by identifying translocation events before and after reactions with nanomolar concentrations of the target sample. Furthermore, this assay was also carried out by using larger size nanopores (60 nm diameter) to achieve the DNAzyme cleavage sensing with the same sample concentration. This approach can improve event detectability of other smaller molecules' translocation, which opens up a wide range of applications for analytes detection by incorporating solid-state na...Continue Reading

References

Aug 25, 2004·Biophysical Journal·Jiunn B HengGregory Timp
Sep 24, 2005·Nano Letters·Arnold J StormCees Dekker
Jan 11, 2007·Journal of the American Chemical Society·Yi XiaoKevin W Plaxco
Nov 22, 2008·Science·John EidStephen Turner
Apr 8, 2009·Nature Nanotechnology·James ClarkeHagan Bayley
Oct 26, 2010·Nature Nanotechnology·Meni WanunuMarija Drndić
Apr 19, 2011·Analytical Chemistry·Wen-Jie LanHenry S White
Sep 20, 2011·Nature Nanotechnology·Bala Murali Venkatesan, Rashid Bashir
Oct 15, 2011·Journal of the American Chemical Society·Shuang WenHai-Chen Wu
Feb 16, 2012·Nature Biotechnology·Gerald M CherfMark Akeson
Jan 19, 2013·Analytical Chemistry·Deanpen JaprungJoshua B Edel
Mar 12, 2013·Nature Nanotechnology·David Rodriguez-Larrea, Hagan Bayley
Apr 26, 2013·Accounts of Chemical Research·Guihua WangXiyun Guan
Jan 2, 2014·Accounts of Chemical Research·Hao PeiChunhai Fan
May 8, 2014·ACS Applied Materials & Interfaces·Alireza AbiXiaolei Zuo
Aug 26, 2015·Analytical Chemistry·Mikiembo Kukwikila, Stefan Howorka
Sep 22, 2015·Nature Nanotechnology·Jiandong FengAleksandra Radenovic
Nov 19, 2015·ACS Nano·Spencer CarsonCarlos A Aguilar
Mar 30, 2016·Chemical Communications : Chem Comm·Yong-Xu HuYi-Tao Long
Apr 5, 2016·Nature Nanotechnology·Nicholas A W Bell, Ulrich F Keyser
Apr 28, 2016·Analytical Chemistry·Chan CaoYi-Tao Long
Aug 11, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Sébastien BalmeJean-Marc Janot
Dec 4, 2016·Biosensors & Bioelectronics·Qiu-Mei FengHong-Yuan Chen
Dec 20, 2016·Nature Nanotechnology·Erik C YuskoMichael Mayer
Feb 17, 2017·Analytical Chemistry·Xiangmeng QuHao Pei
Jun 24, 2017·Nanoscale Research Letters·Libo ZhuQuanjun Liu
Jul 20, 2017·Chemical Communications : Chem Comm·Yi-Lun YingYi-Tao Long

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Citations

Feb 23, 2020·Nucleic Acids Research·Taoli DingCheng Zhang
Oct 22, 2020·Sensors·Imogen Heaton, Mark Platt
Dec 29, 2020·Small Methods·Golbarg Mohammadi RoozbahaniXiyun Guan
May 25, 2021·Frontiers in Chemistry·Hongcheng YangRui Hao

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