Solid-state NMR structure determination of melittin in a lipid environment

Biophysical Journal
Y H LamF Separovic

Abstract

Solid-state (13)C NMR spectroscopy was used to investigate the three-dimensional structure of melittin as lyophilized powder and in ditetradecylphosphatidylcholine (DTPC) membranes. The distance between specifically labeled carbons in analogs [1-(13)C]Gly3-[2-(13)C]Ala4, [1-(13)C]Gly3-[2-(13)C]Leu6, [1-(13)C]Leu13-[2-(13)C]Ala15, [2-(13)C]Leu13-[1-(13)C]Ala15, and [1-(13)C]Leu13-[2-(13)C]Leu16 was measured by rotational resonance. As expected, the internuclear distances measured in [1-(13)C]Gly3-[2-(13)C]Ala4 and [1-(13)C]Gly3-[2-(13)C]Leu6 were consistent with alpha-helical structure in the N-terminus irrespective of environment. The internuclear distances measured in [1-(13)C]Leu13-[2-(13)C]Ala15, [2-(13)C]Leu13-[1-(13)C]Ala15, and [1-(13)C]Leu13-[2-(13)C]Leu16 revealed, via molecular modeling, some dependence upon environment for conformation in the region of the bend in helical structure induced by Pro14. A slightly larger interhelical angle between the N- and C-terminal helices was indicated for peptide in dry or hydrated gel state DTPC (139 degrees -145 degrees ) than in lyophilized powder (121 degrees -139 degrees ) or crystals (129 degrees ). The angle, however, is not as great as deduced for melittin in aligned bilayer...Continue Reading

References

Jun 1, 1979·FEBS Letters·J C TalbotC Lussan
Aug 7, 1979·Biochimica Et Biophysica Acta·A F Drake, R C Hider
Sep 18, 1979·Biochemistry·E KnöppelW Wickner
Jun 16, 1978·Biochimica Et Biophysica Acta·C R DawsonR C Hider
May 7, 1990·Biochimica Et Biophysica Acta·C E Dempsey
Jan 1, 1990·Proteins·J P SegrestG M Anantharamaiah
Jun 20, 1990·Journal of Molecular Biology·R HendersonK H Downing
Apr 1, 1989·International Journal of Peptide and Protein Research·C G FieldsT A Cross
Apr 5, 1988·European Journal of Biochemistry·R BazzoI D Campbell
Jun 5, 1988·Journal of Molecular Biology·D J Barlow, J M Thornton
Oct 1, 1986·Biophysical Journal·H Vogel, F Jähnig
Oct 1, 1981·Biophysical Journal·M T Tosteson, D C Tosteson
Jun 5, 1982·Journal of Molecular Biology·J Warwicker, H C Watson
Aug 19, 1994·Journal of Molecular Biology·R SmithA Makriyannis
Jun 1, 1996·The Biochemical Journal·D E RivettJ A Werkmeister
Feb 1, 1996·Journal of Molecular Graphics·R KoradiK Wüthrich
Nov 21, 1997·Journal of Molecular Biology·P GüntertK Wüthrich
Mar 29, 2000·Biophysical Journal·J H Lin, A Baumgaertner
Apr 24, 2002·Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics·D B LanglaisJ H Davis

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Citations

Sep 12, 2002·Journal of Physiology and Biochemistry·A RochaJ M Coll
Aug 14, 2010·The Journal of Membrane Biology·Alexey S LadokhinStephen H White
Apr 9, 2004·European Biophysics Journal : EBJ·H Raghuraman, Amitabha Chattopadhyay
Aug 2, 2003·Current Opinion in Cell Biology·Khashayar Farsad, Pietro De Camilli
Dec 5, 2002·Current Opinion in Structural Biology·Lynmarie K Thompson
Aug 12, 2004·Chemical Reviews·Stanley J Opella, Francesca M Marassi
Aug 5, 2004·The Journal of Biological Chemistry·Fernando PorcelliGianluigi Veglia
Jul 16, 2013·Biophysical Chemistry·Ummul Liha Khatun, Chaitali Mukhopadhyay
Sep 10, 2014·Amino Acids·Elaheh JamasbiMohammed Akhter Hossain
Oct 20, 2009·FEBS Letters·Guillaume Drin, Bruno Antonny
Dec 9, 2008·Biochimica Et Biophysica Acta·Hyung-Sik WonBong-Jin Lee
Apr 17, 2007·Biophysical Journal·Geert van den BogaartBert Poolman
Jun 9, 2015·Biochimica Et Biophysica Acta·Elaheh JamasbiFrances Separovic
Jul 25, 2015·The Journal of Physical Chemistry. B·Chenyi LiaoJianing Li
Dec 2, 2006·Bioscience Reports·H Raghuraman, Amitabha Chattopadhyay
Mar 17, 2010·International Journal of Antimicrobial Agents·R RasulM D P Willcox
Mar 1, 2011·Biophysical Journal·Andrew C RapsonMichelle L Gee
Sep 5, 2006·Biochimica Et Biophysica Acta·August AnderssonLena Mäler
Jul 15, 2009·Biochimica Et Biophysica Acta·Henrik BiverståhlLena Mäler
Mar 27, 2007·Biochimica Et Biophysica Acta·Mojca MallyBostjan Zeks
Nov 6, 2007·The Journal of Chemical Physics·Atanu Das, Chaitali Mukhopadhyay
Dec 13, 2018·Physical Chemistry Chemical Physics : PCCP·Hannah M BrittJohn M Sanderson
Jul 28, 2009·Magnetic Resonance in Chemistry : MRC·Robert C Neuman, John T Gerig
Mar 17, 2017·Annual Review of Analytical Chemistry·Renee J TranJohn C Conboy
Oct 27, 2017·Chemistry : a European Journal·Marc-Antoine Sani, Frances Separovic
Mar 3, 2019·Applied Microbiology and Biotechnology·Hamed MemarianiHamideh Moravvej
Jan 26, 2020·International Journal of Molecular Sciences·Thiru SabapathyRicardo L Mancera
Jan 29, 2011·Chemistry, an Asian Journal·Sekar Karuppannan, Jean-Claude Chambron
Sep 4, 2009·The Journal of Physical Chemistry. B·Michal Gordon-GrossmanDaniella Goldfarb
Dec 14, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Zubaidah NingsihMichelle L Gee
Aug 18, 2018·The Journal of Physical Chemistry. B·Stephen J FoxChandra S Verma

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