Solid state self-assembly mechanism of RADA16-I designer peptide

Biomacromolecules
Ashley R CormierAnant K Paravastu

Abstract

We report that synthetic RADA16-I peptide transforms to β-strand secondary structure and develops intermolecular organization into β-sheets when stored in the solid state at room temperature. Secondary structural changes were probed using solid state nuclear magnetic resonance spectroscopy (ssNMR) and Fourier transform infrared spectroscopy (FTIR). Intermolecular organization was analyzed via wide-angle X-ray diffraction (WAXD). Observed changes in molecular structure and organization occurred on the time scale of weeks during sample storage at room temperature. We observed structural changes on faster time scales by heating samples above room temperature or by addition of water. Analysis of hydration effects indicates that water can enhance the ability of the peptide to convert to β-strand secondary structure and assemble into β-sheets. However, temperature dependent FTIR and time dependent WAXD data indicate that bound water may hinder the assembly of β-strands into β-sheets. We suggest that secondary structural transformation and intermolecular organization together produce a water-insoluble state. These results reveal insights into the role of water in self-assembly of polypeptides with hydrophilic side chains, and have imp...Continue Reading

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Citations

Aug 28, 2013·ACS Nano·Ashley R CormierAnant K Paravastu
Jun 17, 2015·Clinical and Translational Science·Fei-Fan XuGilberto K K Leung
Dec 25, 2015·Biomaterials Science·Zhiqiang YuShuwen Liu
Jun 27, 2013·Journal of Peptide Science : an Official Publication of the European Peptide Society·Ashley R CormierAnant K Paravastu
Jun 12, 2016·Nanomedicine : Nanotechnology, Biology, and Medicine·Xiaoxiao WangYaojiong Wu
May 26, 2017·PloS One·Sibel CetinelCarlo Montemagno
Oct 4, 2019·Journal of Biomedical Materials Research. Part a·Yi WangJingfeng Li
Apr 26, 2019·Journal of Biomaterials Science. Polymer Edition·Rongrong WangZhuoyue Chen
Feb 11, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Shuda WeiChangsheng Liu

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