Solid surface fluorescence immunosensor for ultrasensitive detection of hepatitis B virus surface antigen using PAMAM/CdTe@CdS QDs nanoclusters

Methods and Applications in Fluorescence
Bahareh BabamiriAbdollah Salimi

Abstract

In the present study, we constructed an ultrasensitive solid surface fluorescence-immunosensor based on highly luminescent CdTe@CdS-PAMAM structures as nanoprobe for determination of HBsAg by monitoring fluorescence intensity. This strategy was achieved by using PAMAM as a signal amplifier; the PAMAM dendrimer with the many functional amine groups can amplify the fluorescence signal of QDs by covalent attachment of CdTe@CdS on PAMAM and hence, improve the sensitivity of the proposed method significantly. A sandwich type immunosensor was formed after the addition of HBsAg and the PAMAM-QD-Ab2, respectively. Under optimal conditions, the designed immunosensor demonstrates a good analytical performance for the HBsAg detection in an excellent linear range from 5 fg ml-1 to 0.15 ng ml-1 with the detection limit (LOD) of 0.6 fg ml-1 at a S/N ratio of 3. In addition, the analysis of human serum samples shows that the fluorescent immunoassay has the great potential for early diagnosis of hepatitis B and can be used for the detection of other tumor markers in clinical applications.

References

Apr 14, 1999·CA: a Cancer Journal for Clinicians·D M ParkinJ Ferlay
Sep 14, 2007·Journal of the American Chemical Society·Ewa M GoldysZygmunt Gryczynski
Aug 30, 2008·Nature Methods·Ute Resch-GengerThomas Nann
Apr 14, 2010·Chemical Communications : Chem Comm·Jiaofang LiuLinyong Zhu
Jul 15, 2011·Luminescence : the Journal of Biological and Chemical Luminescence·Huaizhen HeLangchong He
Oct 15, 2011·The Journal of Physical Chemistry. B·Michael R MankbadiJohn N Kuhn
Jan 6, 2012·Chemical Communications : Chem Comm·Qinghui ZengHong Zhang
Jun 21, 2012·Luminescence : the Journal of Biological and Chemical Luminescence·Guan-Feng LinYing-Song Wu
Feb 23, 2013·Biosensors & Bioelectronics·Zhenzhen ChenFangqiong Tang
Feb 18, 2014·Clinica Chimica Acta; International Journal of Clinical Chemistry·Fei FanXixiong Kang
May 1, 2016·Journal of Infection in Developing Countries·Tristan BanksRose McGready

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