Solubility of palmitoyl-coenzyme A in acyltransferase assay buffers containing magnesium ions

Archives of Biochemistry and Biophysics
P P Constantinides, J M Steim

Abstract

The solubility of palmitoyl-CoA is strongly affected by Mg2+ concentrations commonly used in acyltransferase reactions. In 0.10 M Tris-HCl buffer at pH 7.4 or 8.5, all of the palmitoyl-CoA in 10 microM solutions and 90% of the palmitoyl-CoA in 100 microM solutions are precipitated by 1 mM Mg2+. In 0.05 M phosphate at pH 7.4, and in 0.10 M Tris-HCl containing 0.4 M KCl, the substrate remains soluble at Mg2+ concentrations below 4-5 mM. Above 5 mM Mg2+, palmitoyl-CoA is insoluble in all of these buffers. Substrate solubility could therefore be a limiting factor when free Mg2+ and fatty acyl-CoAs are present together during acyltransferase assays.

References

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Citations

Mar 1, 1988·Archives of Biochemistry and Biophysics·P P Constantinides, J M Steim
Jan 22, 2014·Analytical Biochemistry·Andrew MalinaS Douglass Gilman
Oct 15, 2013·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Daiki SetoyamaDaisuke Miura
Nov 1, 2007·The Journal of Biological Chemistry·Eliza PloskońMatthew P Crump
Aug 31, 1987·Biochemical and Biophysical Research Communications·M J StasiaP V Vignais

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