journal cover

Solubilization and characterization of a low-affinity histamine-binding site on human blood mononuclear cells

Molecular Immunology

Apr 1, 1986

R S WarlowC C Bernard

Abstract

The extract of human peripheral blood lymphocytes and monocytes treated with Triton X-100, in direct- and competitive-binding studies, with 10(-6)-10(-2) M [14C]histamine contained a low-affinity binding site whose dissociation constant (Kd 1.8 X 10(-4) M) was commensurate with the conc...read more

Mentioned in this Paper

Histamine Measurement
Cations, Divalent
Mononuclear Cells
Mast Cell
Peripheral Blood
Calcium
Lymphocytes as Percentage of Blood Leukocytes (Lab Test)
Lymphoid Cells
Histamine Receptor
Sulfhydryl Compounds
Paper Details
References
  • References21
  • Citations6
123

Solubilization and characterization of a low-affinity histamine-binding site on human blood mononuclear cells

Molecular Immunology

Apr 1, 1986

R S WarlowC C Bernard

PMID: 3088433

DOI: 10.1016/0161-5890(86)90137-9

Abstract

The extract of human peripheral blood lymphocytes and monocytes treated with Triton X-100, in direct- and competitive-binding studies, with 10(-6)-10(-2) M [14C]histamine contained a low-affinity binding site whose dissociation constant (Kd 1.8 X 10(-4) M) was commensurate with the conc...read more

Mentioned in this Paper

Histamine Measurement
Cations, Divalent
Mononuclear Cells
Mast Cell
Peripheral Blood
Calcium
Lymphocytes as Percentage of Blood Leukocytes (Lab Test)
Lymphoid Cells
Histamine Receptor
Sulfhydryl Compounds

Related Papers

Paper Details
References
  • References21
  • Citations6
123
/papers/solubilization-and-characterization-of-a-low-affin/3088433