PMID: 3760878Nov 1, 1986Paper

Solubilization and characterization of D2-dopamine receptors in an estrone-induced, prolactin-secreting rat pituitary adenoma

Journal of Neurochemistry
C BouvierR Collu

Abstract

D2-dopamine (3,4-dihydroxyphenylethylamine) receptors were successfully solubilized with 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate from an estrone-induced rat pituitary adenoma. Forty-five percent of initial protein and 48% of initial [3H]spiroperidol binding sites were solubilized. The high affinity as well as the stereoselectivity of the sites was preserved. The order of potency of dopaminergic agonists was found to be typical of D2 receptors. Target size analysis by radiation inactivation indicated a molecular weight of 143,000 +/- 3,000 and of 106,000 +/- 4,000 daltons for membrane-bound and solubilized receptors, respectively. This suggests the loss of a 37,000-dalton subunit during solubilization without significant modification of binding characteristics. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of receptor protein preparation photolabeled with N-(p-azido-m[125I]iodophenethyl)spiroperidol confirmed the existence of a 94,000-dalton peptide which probably constitutes the ligand binding site of the receptor. Thus, our data indicate that chronic estrogen treatment of rats, although inducing a pituitary adenoma, does not modify the pharmacological characteristics of D2 receptors. These data ...Continue Reading

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Citations

Nov 1, 1987·Molecular and Cellular Endocrinology·H B Niznik
Jun 1, 1991·Molecular and Cellular Endocrinology·C BouvierR Collu
Jun 17, 2000·Trends in Endocrinology and Metabolism : TEM·A SalahpourM Bouvier
Dec 15, 2004·Biochemistry·Paul S-H ParkKrzysztof Palczewski
Aug 1, 1994·Journal of Neuroendocrinology·J C PainsonR Collu
Apr 3, 2001·Nature Reviews. Neuroscience·M Bouvier
Jul 17, 1998·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·T E Hébert, M Bouvier

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