Apr 25, 1976

Solubilization and characterization of the beta-adrenergic receptor binding sites of frog erythrocytes

The Journal of Biological Chemistry
M G Caron, R J Lefkowitz

Abstract

Specific beta-adrenergic receptors present in membrane preparations of frog erythrocytes were identified by binding of (-)-[3H]dihydroalprenolol, a potent competitive beta-adrenergic antagonist. The (-)-[3H]dihydroalprenolol binding sites could be solubilized by treatment of a purified erythrocyte membrane fraction with the plant glycoside digitonin but not by treatment with a wide variety of other detergents. The binding sites appeared to be soluble by several independent experimental criteria including (a) failure to sediment of 105,000 X g for 2 hours; (b) passage through 0.22-mu Millipore filters; (c) chromatography on Sepharose 6B gels; and (d) electron microscopy. The soluble receptor sites retained all of the essential characteristics of the membrane-bound sites, namely rapid and reversible binding of beta-adrenergic agonists and antagonists; strict stereospecificity toward both beta-adrenergic agonists and antagonists; appropriate structure-activity relationships; saturability of the sites at low concentrations of ligand; no affinity for alpha-adrenergic drugs, nonphysiologically active catechol compounds, and catecholamine metabolites. Based on gel chromatography in the presence of detergent, the molecular weight of th...Continue Reading

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Mentioned in this Paper

Rana pipiens
Tryptophan
Deoxyribonuclease I
Tissue Membrane
Catecholamine [EPC]
Structure-Activity Relationship
Peptide Hydrolases
Chromatography
Digitonin
Phospholipase A Activity

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