Solubilization and chemical characterization of an insoluble matrix protein in the gastroliths of a crayfish, Procambarus clarkii

Bioscience, Biotechnology, and Biochemistry
K IshiiH Nagasawa

Abstract

The gastrolith of the crayfish Procambarus clarkii contains a small amount of an organic matrix that is mainly chitin and proteins, together with a large amount of calcium carbonate. As the first step to understand the mechanism of calcification, we tried to characterize matrix proteins in the gastrolith. An insoluble matrix protein, referred to as gastrolith matrix protein, was made soluble with 1% SDS containing 10 mM dithiothreitol, and was purified by reverse-phase high-performance liquid chromatography. The protein had a molecular weight of about 50,500 and a blocked amino terminus. By enzymatic digestion and microsequencing, five partial amino acid sequences with a total of 225 amino acid residues were identified and found to include a repetitive sequence not reported previously.

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Citations

Oct 8, 2013·Bioscience, Biotechnology, and Biochemistry·Hiromichi Nagasawa
Jan 18, 2014·Proceedings of the National Academy of Sciences of the United States of America·Laura M HammPatricia M Dove
Feb 13, 2010·The Journal of Biological Chemistry·Lilah GlazerAmir Sagi
Aug 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Anat Akiva-TalAsher Schmidt
May 11, 2010·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Yana YudkovskiMoshe Tom
Sep 23, 2008·Micron : the International Research and Review Journal for Microscopy·Hermann EhrlichOleg S Pokrovsky
Aug 16, 2015·Journal of Structural Biology·M S FernándezJ L Arias
May 23, 2006·Biophysical Journal·Yaowu XiaoM Shane Hutson
Apr 14, 2021·Scientific Reports·Eri NakamuraHiroaki Imai
Jun 7, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·T WatanabeM Kono

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