Solubilization of a stereospecific opiate-macromolecular complex from rat brain.

Science
E J SimonI Edelman

Abstract

A [3H]etorphine-macromolecular complex has been solubilized from rat brain synaptosomal fraction by extraction with the nonionic detergent Brij 36T. Stereospecificity of binding to this solubilized complex was demonstrated by the finding that radioactivity in the complex was virtually eliminated when binding had occurred in the presence of excess levorphanol, an active narcotic analgesic, while it was unaffected by its inactive enantiomorph dextrorphan. Bound radioactivity was dissociated by proteolytic enzymes, sulfhydryl reagents, and heat, suggesting the presence of protein. The bound solubilized macromolecular moiety may be the opiate receptor.

References

Jul 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·E J SimonI Edelman
Mar 9, 1973·Science·C B Pert, S H Snyder
Aug 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·A GoldsteinB K Pal

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Citations

Sep 1, 1983·Naunyn-Schmiedeberg's Archives of Pharmacology·S OttM Wüster
Feb 1, 1976·Neurochemical Research·E J Simon
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Jan 1, 1980·Annals of the New York Academy of Sciences·M Weisenberg
Jun 22, 1981·Biochimica Et Biophysica Acta·E J Victoria, L C Mahan

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