PMID: 7536473Apr 12, 1995Paper

Solubilization of glycosyl-phosphatidylinositol-anchored proteins in quiescent and stimulated neutrophils

Biochimica Et Biophysica Acta
T J CainJ M Robinson

Abstract

In human neutrophils, alkaline phosphatase (AlkPase), a low-affinity receptor for IgG (FcRIIIB), and complement decay accelerating factor (DAF) are glycosyl-phosphatidylinositol (GPI)-anchored proteins. Varying greatly in biological function these three integral membrane proteins exhibit regulated cell surface expression in neutrophils. Defined by their common membrane-linkage motif, AlkPase, FcRIIIB, and DAF can be released from the lipid bilayer by the action of phosphatidylinositol-specific phospholipase C and are relatively resistant to low temperature extraction with Triton X-100 (TX-100). In this study we show that neutrophil AlkPase, FcRIII, and DAF display differential extractibility; they are relatively insensitive to TX-100 solubilization at 4 degrees C, but are readily extracted with TX-100 at 37 degrees C or by the detergent octyl glucoside at 4 degrees C. The differential extractibility of these GPI-anchored proteins is the same in unstimulated cells, where these proteins exist primarily in an intracellular pool, and stimulated cells, where they are expressed principally at the cell surface. However, no differential extraction effect is observed with two neutrophil transmembrane proteins, complement receptor 1 (CD3...Continue Reading

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Citations

Oct 6, 1998·Annual Review of Biochemistry·R G Anderson
Jun 5, 2004·Peptides·Lai-San SiuEl-Nasir Lalani
Oct 21, 2006·Biochemical and Biophysical Research Communications·Rick F ThorneGordon F Burns
Sep 26, 2002·Journal of Biochemistry and Molecular Biology·Chong-Ho KimPatrick C Elwood
Mar 11, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·T TakizawaJ M Robinson
Mar 19, 2004·The Journal of Biological Chemistry·Jeremy J RoseSundararajan Venkatesan
Oct 25, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·P PeyronI Maridonneau-Parini
Aug 6, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Toshihiro TakizawaJohn M Robinson
Nov 5, 1997·Biochimica Et Biophysica Acta·O NosjeanB Roux

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