PMID: 39541May 1, 1979

Solubilization of human erythrocyte membrane glycoproteins by triton X-100

The Biochemical Journal
R S Pratt, G M Cook

Abstract

1. The enzymic removal of sialic acid residues from the glycoproteins of the human erythrocyte decreases the solubilization of membrane glycoprotein by Triton X-100. 2. The solubilization of asialoglycoprotein by Triton X-100 may be restored by the addition of borate. 3. Use of this non-ionic detergent in the presence of borate, as a general procedure for the mild solubilization of membrane glycoproteins deficient in sialic acid residues, is discussed.

Related Concepts

Glycoproteins
Sialic Acid Assay
Neoglycoproteins
Asialoglycoproteins
Membrane Glycoproteins
Vigilon
Borates
polysaccharide-K
Red Cell Ghost
Triton X-100

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