Solubilization of proteins in extracted oil bodies by SDS: a simple and efficient protein sample preparation method for Tricine-SDS-PAGE

Food Chemistry
Yusang YingYeming Chen

Abstract

A simple and efficient method for preparing Tricine-SDS-PAGE protein sample of extracted oil bodies (OBs) was supplied: OB suspension was vortexed with SDS buffer (pH 6.8) for 2 min at room temperature with SDS/protein of 1.52/1(w/w), which could be analyzed by Tricine-SDS-PAGE after simple treatments (dilution and 2-mercaptoethanol). At SDS/protein of 1.52/1, about 95% of proteins in soybean OB suspension were solubilized, whereas residual 5% of proteins were weakly bound to SDS-destroyed OBs; proteins in destroyed OBs might be further solubilized by SDS in the gel and cathode buffer of Tricine-SDS-PAGE, causing about 99% of proteins in soybean OB suspension recover on Tricine-SDS-PAGE gel, which was better than acetone (89%) and diethyl ether (96%) harvested protein samples. Higher or lower SDS/protein was unbeneficial for protein solubilization from OBs. Additionally, the above method was also better than organic solvent method for peanut, sesame, and rapeseed OB suspensions.

References

Apr 1, 1992·The Journal of Cell Biology·J T Tzen, A H Huang
Jul 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·J A Reynolds, C Tanford
Nov 25, 2000·Biochimica Et Biophysica Acta·M le MaireJ V Moller
Nov 3, 2004·Biochimica Et Biophysica Acta·Annela M SeddonPaula J Booth
Oct 4, 2005·Plant Physiology and Biochemistry : PPB·Li-Jen LinJason T C Tzen
Apr 5, 2007·Nature Protocols·Hermann Schägger
Sep 21, 2007·Journal of Agricultural and Food Chemistry·Daigo IwanagaDavid Julian McClements
Feb 7, 2008·Journal of Agricultural and Food Chemistry·Virginie N KapchiePatricia A Murphy
Jun 3, 2008·Journal of Agricultural and Food Chemistry·Carmen Martín-HernándezLadislav Obert
Jun 30, 2009·Proteomics·Pascale JolivetNathalie Nesi
Jun 1, 2010·Journal of Agricultural and Food Chemistry·Yeming Chen, Tomotada Ono
Apr 9, 2013·Food Chemistry·Olga A KarkaniVassilis Kiosseoglou
Sep 14, 2013·Journal of Agricultural and Food Chemistry·Luping ZhaoYufei Hua
Jan 23, 2014·Journal of Agricultural and Food Chemistry·Yeming ChenYufei Hua

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Citations

Dec 7, 2014·Plant Science : an International Journal of Experimental Plant Biology·Xiao WangBernd Wollenweber
Jun 29, 2017·Journal of Agricultural and Food Chemistry·Luping ZhaoYeming Chen
Nov 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yuemei ZhangYapeng Fang
Jun 3, 2020·Journal of Food Science·Longzheng ZhouLianzhou Jiang
Mar 18, 2020·Journal of Colloid and Interface Science·Nan YangGlyn O Phillips

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