PMID: 11607356Jan 15, 1993Paper

Solubilized proteins from carrot (Daucus carota L.) membranes bind calcium channel blockers and form calcium-permeable ion channels

Proceedings of the National Academy of Sciences of the United States of America
P ThuleauJ I Schroeder

Abstract

Calcium channels have been suggested to play a major role in the initiation of a large number of signal transduction processes in higher plant cells. However, molecular components of higher plant Ca2+ channels remain unidentified to date. Calcium channel blockers of the phenylalkylamine family and bepridil specifically inhibit Ca2+ influx into carrot (Daucus carota L.) cells. By using a phenylalkylamine azido derivative, a 75-kDa carrot membrane protein has been previously identified. Here we have partially purified this Ca2+ channel blocker-binding protein by lectin-affinity and ion-exchange chromatographies. The protein fraction containing the 75-kDa binding protein was incorporated into giant liposomes. Single-channel patch-clamp studies on these proteoliposomes showed the presence of Ca2+-permeable channel currents. These Ca2+-permeable channels were not stable. Recordings after durations of 2-10 min showed the appearance of nonselective ion channels with a permeability to calcium and chloride ions. These nonselective Ca2+-permeable ion channels, in contrast, were stable and were recorded for extended durations. The addition of the Ca2+ channel-blocker bepridil (10 M) led to the inhibition of these nonselective Ca2+-permeab...Continue Reading

References

Jul 1, 1992·International Journal of Radiation Biology·C L SandersJ A Mahaffey
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J I Schroeder, S Hagiwara
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W A HorneR A Cerione
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Dec 15, 1990·Proceedings of the National Academy of Sciences of the United States of America·P ThuleauR Ranjeva
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J I SchroederE Neher
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P ThuleauR Ranjeva

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Citations

Apr 5, 2000·Biochimica Et Biophysica Acta·P J White
Aug 17, 2002·Molecular Plant-microbe Interactions : MPMI·Frederic GressentJean-Jacques Bono
Sep 29, 2001·Plant & Cell Physiology·T FuruichiS Muto
May 23, 2018·International Journal of Molecular Sciences·Kjell De VrieseSteffen Vanneste

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