Soluble adenylyl cyclase: A novel player in cardiac hypertrophy induced by isoprenaline or pressure overload

PloS One
Ilona SchirmerKornelia Jaquet

Abstract

In contrast to the membrane bound adenylyl cyclases, the soluble adenylyl cyclase (sAC) is activated by bicarbonate and divalent ions including calcium. sAC is located in the cytosol, nuclei and mitochondria of several tissues including cardiac muscle. However, its role in cardiac pathology is poorly understood. Here we investigate whether sAC is involved in hypertrophic growth using two different model systems. In isolated adult rat cardiomyocytes hypertrophy was induced by 24 h β1-adrenoceptor stimulation using isoprenaline (ISO) and a β2-adrenoceptor antagonist (ICI118,551). To monitor hypertrophy cell size along with RNA/DNA- and protein/DNA ratios as well as the expression level of α-skeletal actin were analyzed. sAC activity was suppressed either by treatment with its specific inhibitor KH7 or by knockdown. Both pharmacological inhibition and knockdown blunted hypertrophic growth and reduced expression levels of α-skeletal actin in ISO/ICI treated rat cardiomyocytes. To analyze the underlying cellular mechanism expression levels of phosphorylated CREB, B-Raf and Erk1/2 were examined by western blot. The results suggest the involvement of B-Raf, but not of Erk or CREB in the pro-hypertrophic action of sAC. In wild type and...Continue Reading

References

Mar 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·T Braun, R F Dods
Jan 1, 1991·Methods in Enzymology·T Braun
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·T C HeB Vogelstein
Jan 6, 1999·Proceedings of the National Academy of Sciences of the United States of America·J BuckL R Levin
Jul 27, 2000·American Journal of Physiology. Cell Physiology·M SchäferK D Schlüter
Jan 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Christopher L AntosEric N Olson
Apr 18, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Yulin LiaoMasatsugu Hori
Dec 12, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jonathan H ZippinJochen Buck
Mar 1, 2003·The Journal of Biological Chemistry·Tatiana N LitvinLonny R Levin
Feb 11, 2004·The Journal of Cell Biology·Jonathan H ZippinJochen Buck
Jul 28, 2004·Cardiovascular Research·Tong Zhang, Joan Heller Brown
Jan 22, 2005·American Journal of Physiology. Cell Physiology·Weidong GengOrson W Moe
May 16, 2006·Basic Research in Cardiology·Georg BaumgartenPascal Knuefermann
Feb 20, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Marek MatusFrank U Müller
May 23, 2007·Journal of Cardiac Failure·Kenneth P RoosKenneth D Philipson
Mar 8, 2008·Circulation Research·Mélanie MétrichFrank Lezoualc'h
Jun 13, 2008·Pflügers Archiv : European journal of physiology·Stephanie ReisKornelia Jaquet
Dec 30, 2008·Heart Failure Reviews·Ali El-Armouche, Thomas Eschenhagen
Mar 4, 2009·Cell Metabolism·Rebeca Acin-PerezGiovanni Manfredi
Apr 2, 2009·The Journal of Biological Chemistry·Sanjeev KumarYury Ladilov
Aug 5, 2010·Pflügers Archiv : European journal of physiology·Martin TresguerresLonny R Levin
Aug 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Chang Hoon HaZheng Gen Jin
Dec 22, 2010·Circulation·Izhak Kehat, Jeffery D Molkentin
Feb 23, 2011·The Journal of Clinical Investigation·Bruce D Gelb, Marco Tartaglia
Apr 15, 2011·Kidney International·Martin TresguerresJochen Buck
Jul 31, 2012·Cardiovascular Research·Marie VidalKristina Lorenz
Oct 5, 2013·The Journal of Pharmacology and Experimental Therapeutics·Jacob L BittermanJochen Buck
Oct 9, 2013·The Journal of Biological Chemistry·Jonathan H ZippinJochen Buck
Dec 11, 2013·Frontiers in Physiology·Nawreen RahmanLonny R Levin
Apr 8, 2014·Pharmacology & Therapeutics·Konstantinos Lefkimmiatis, Manuela Zaccolo
May 31, 2014·American Journal of Respiratory Cell and Molecular Biology·Xi ChenMatthias Salathe
Jul 11, 2014·Biochimica Et Biophysica Acta·Yury Ladilov, Avinash Appukuttan
Sep 27, 2014·Biochimica Et Biophysica Acta·Avinash AppukuttanYury Ladilov
Aug 6, 2015·Circulation Research·Anna ZoccaratoManuela Zaccolo

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Citations

May 13, 2018·Journal of Cardiovascular Development and Disease·Sofya Pozdniakova, Yury Ladilov
Nov 21, 2020·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Mandy LoAnthony P Farrell
Jan 24, 2021·Cardiovascular Research·Yuening LiuZhaokang Cheng
May 1, 2021·Cells·Anne-Sophie Colombe, Guillaume Pidoux

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BETA
transfection
Protein-Assay
transfections

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SigmaPlot
GraphPad Prism
Axio vision
Excel

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