PMID: 8944729Nov 1, 1996Paper

Soluble and insoluble fibronectin increases alveolar epithelial wound healing in vitro

The American Journal of Physiology
C GaratM A Matthay

Abstract

Adhesive interactions between cells and extracellular matrix proteins are important in cell attachment, migration, and proliferation. The present work defines the role of fibronectin (soluble and insoluble) compared with type I and type IV collagen on in vitro alveolar epithelial wound healing. Repeated video microscopy experiments demonstrated that the half-time of wound closure was decreased in the presence of soluble fibronectin (6.6 +/- 2.1 vs. 17.4 +/- 0.8 h in serum-free medium, P < 0.05). Video microscopy, electron microscopy, and vinculin distribution demonstrated the contribution of two main events during the repair process: the migration of epithelial cell sheets and the spreading of the cells. During the wound healing, the internuclear distance between two adjacent cells at the migrating edge of the wound was significantly increased 10 h after wounding in the presence of soluble fibronectin (67 +/- 3.0 vs. 45 +/- 1.5 microns in serum-free medium, P < 0.05), indicating that cell spreading is involved as part of the mechanism for wound closure. Compared with type I and type IV collagen, insoluble fibronectin was the most potent stimulus for alveolar type II cell motility and wound healing in the absence of other serum ...Continue Reading

Citations

Dec 10, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Blandine DoornaertChantal Lafuma
Dec 20, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·D S Allen-GipsonT A Wyatt
Mar 11, 2000·American Journal of Physiology. Cell Physiology·H LeeS An
Oct 17, 2002·Journal of Applied Physiology·J I SznajderD H Ingbar
Aug 10, 2007·American Journal of Physiology. Cell Physiology·Silvina GrassoSilvia Chifflet
Sep 23, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Leena P DesaiChristopher M Waters
Feb 5, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·S BuckleyD Warburton
Nov 15, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·T GeiserM A Matthay
May 9, 2009·American Journal of Respiratory Cell and Molecular Biology·Michael KovalJesse Roman
May 4, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Thomas GeiserV Courtney Broaddus
Sep 14, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Michael J LaFeminaJames A Frank
Jan 15, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Rachel L ZemansGregory P Downey
Mar 10, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·A I Alford, D E Rannels
Jul 3, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Leena P DesaiChristopher M Waters
Mar 18, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Denise C Hocking, Cecilia H Chang
Dec 19, 2020·Clinical and Experimental Pharmacology & Physiology·Rajesh M JagirdarSotirios G Zarogiannis
Oct 6, 2000·Clinics in Chest Medicine·D H Ingbar

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