Soluble bone-derived osteopontin promotes migration and stem-like behavior of breast cancer cells

PloS One
Graciella M PioAlison L Allan

Abstract

Breast cancer is a leading cause of cancer death in women, with the majority of these deaths caused by metastasis to distant organs. The most common site of breast cancer metastasis is the bone, which has been shown to provide a rich microenvironment that supports the migration and growth of breast cancer cells. Additionally, growing evidence suggests that breast cancer cells that do successfully metastasize have a stem-like phenotype including high activity of aldehyde dehydrogenase (ALDH) and/or a CD44+CD24- phenotype. In the current study, we tested the hypothesis that these ALDHhiCD44+CD24- breast cancer cells interact with factors in the bone secondary organ microenvironment to facilitate metastasis. Specifically, we focused on bone-derived osteopontin and its ability to promote the migration and stem-like phenotype of breast cancer cells. Our results indicate that bone-derived osteopontin promotes the migration, tumorsphere-forming ability and colony-forming ability of whole population and ALDHhiCD44+CD24- breast cancer cells in bone marrow-conditioned media (an ex vivo representation of the bone microenvironment) (p≤0.05). We also demonstrate that CD44 and RGD-dependent cell surface integrins facilitate this functional r...Continue Reading

References

May 1, 1992·Clinical & Experimental Metastasis·L Weiss
Jan 1, 1987·British Journal of Cancer·R E Coleman, R D Rubens
Feb 1, 1994·Journal of Cellular Biochemistry·J W XuanA F Chambers
Jan 1, 1996·Annual Review of Cell and Developmental Biology·E Ruoslahti
Dec 17, 1997·International Journal of Cancer. Journal International Du Cancer·M T GillespieD M Findlay
May 16, 1998·International Journal of Cancer. Journal International Du Cancer·T UeE Tahara
Oct 7, 1998·International Journal of Cancer. Journal International Du Cancer·A B TuckA F Chambers
Dec 22, 1999·British Journal of Cancer·F ForozanS P Ethier
Apr 24, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·H NemotoM Noda
Jan 24, 2002·Breast Cancer Research and Treatment·A B TuckA F Chambers
Apr 3, 2002·JAMA : the Journal of the American Medical Association·Jae-Hoon KimSamuel C Mok
May 16, 2002·Journal of Mammary Gland Biology and Neoplasia·A B Tuck, A F Chambers
Mar 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Muhammad Al-HajjMichael F Clarke
Dec 6, 2003·Science·Anne J RidleyAlan Rick Horwitz
Jun 2, 2004·Nature Reviews. Cancer·Klaus Pantel, Ruud H Brakenhoff
Aug 27, 2005·Cell Cycle·David N Haylock, Susan K Nilsson
Mar 7, 2006·Cancer·Kenneth R HessJames L Abbruzzese
Jun 3, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Vivien H C BramwellAnn F Chambers
Jul 4, 2006·The American Journal of Pathology·Alison L AllanAlan B Tuck
Oct 6, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Marija BalicRichard J Cote
Mar 30, 2007·The Journal of Biological Chemistry·Byung-Hoon LeeMelanie H Cobb
Sep 6, 2007·Cell Cycle·Lauren L Campbell, Kornelia Polyak
Jan 10, 2008·Journal of Cellular and Molecular Medicine·A K Croker, A L Allan
Sep 12, 2008·Nature Reviews. Cancer·Jane E Visvader, Geoffrey J Lindeman
Apr 14, 2009·The Journal of Biological Chemistry·Zhaohuan ZhangCheng He
Oct 29, 2010·Journal of the National Comprehensive Cancer Network : JNCCN·Allan LiptonMatthew R Smith
Jan 23, 2010·Cellular and Molecular Life Sciences : CMLS·Sónia Moniz, Peter Jordan
Mar 9, 2010·Current Molecular Medicine·L A ShevdeR S Samant
Nov 30, 2010·Seminars in Oncology·Theresa Guise
Oct 6, 2011·Molecular Cancer Research : MCR·Jeanne M V Louderbough, Joyce A Schroeder
Jun 9, 2012·Journal of Mammary Gland Biology and Neoplasia·Lauren M RotaTeresa L Wood
Sep 19, 2012·Journal of Signal Transduction·Yannick D BenoitJean-François Beaulieu

❮ Previous
Next ❯

Citations

Nov 23, 2018·Journal of Tissue Engineering and Regenerative Medicine·Sumanta KarKalpana S Katti
Aug 23, 2019·Cancer Biotherapy & Radiopharmaceuticals·Xiaofei XuMingqing Shi
Sep 11, 2019·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Mourad AssidiJaudah Al-Maghrabi
Mar 22, 2019·International Journal of Environmental Research and Public Health·Sung Min NamByung-Joon Chang
Dec 30, 2020·Cancers·Flavia Fico, Albert Santamaria-Martínez
May 11, 2021·Frontiers in Oncology·Amrutha MohanTessy Thomas Maliekal
Sep 16, 2019·International Immunopharmacology·Laleh SharifiMonireh Mohsenzadegan
Jul 14, 2021·Molecular Cancer Therapeutics·Ankita B JaykumarMelanie H Cobb

❮ Previous
Next ❯

Methods Mentioned

BETA
protein assay
Protein Array
ELISA
flow
flow cytometry
transfection
FACS

Software Mentioned

Protein Array Analyzer for ImageJ
GraphPad
Image J
GraphPad Prism
ImageJ
[UNK]
RayBiotech

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Breast Invasive Carcinoma (Keystone)

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

Breast Invasive Carcinoma

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.