PMID: 8603996Feb 1, 1996Paper

Soluble CD14 and lipopolysaccharide-binding protein from bovine serum enable bacterial lipopolysaccharide-mediated cytotoxicity and activation of bovine vascular endothelial cells in vitro

Journal of Leukocyte Biology
Z YangP N Bochsler

Abstract

Bacterial endotoxin (lipopolysaccharide, LPS) has potent proinflammatory properties toward many cell types, including vascular endothelial cells. Bovine endothelial cells are often used for investigations involving the vascular endothelium in vitro, and other bovine products such as fetal bovine serum are also widely utilized in research laboratories. Evidence is presented that soluble CD14 (sCD14) is present in bovine serum and that LPS-mediated activation and cytotoxicity to bovine endothelial cells in vitro are dependent on sCD14. LPS-mediated activation of endothelial cells was quantitated by measuring tissue factor expression using an activated factor X-related chromogenic assay. Concentrations of 0.1-5.0% fetal bovine serum in the culture medium promoted LPS-induced tissue factor expression on bovine endothelial cells, and anti-CD14 monoclonal antibody (mAb) (20 micrograms/ml) inhibited tissue factor expression, whereas control antibodies did not. LPS-mediated damage to endothelial cells was assayed using the MTT tetrazolium assay. We found that either serum or recombinant human soluble CD14 (rsCD14, 20-2000 ng/ml) was required for LPS-related endothelial cell damage and that anti-CD14 mAb inhibited cytotoxicity. In addit...Continue Reading

Citations

Aug 8, 1997·Journal of Toxicology and Environmental Health·P R Mayeux
Apr 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ian SabroeSteven K Dower
Sep 22, 2006·Histochemistry and Cell Biology·Sandrine Bès-HoutmannMaya Cesari
Mar 20, 2008·Allergology International : Official Journal of the Japanese Society of Allergology·Takao FujisawaYoshiko Kato
Apr 22, 2016·Journal of Virology·Nadine van MontfoortAndrea M Woltman
Feb 20, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A Kittel
May 9, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Douglas D Bannerman, Simeon E Goldblum
Aug 13, 2002·The Journal of Biological Chemistry·Salamatu S MambulaStuart M Levitz
Nov 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Przemyslaw LewkowiczHenryk Tchórzewski
May 8, 2019·Cellular and Molecular Life Sciences : CMLS·Malvina PizzutoClare E Bryant
Aug 24, 2002·The Journal of Biological Chemistry·Michel L VandenplasJames N Moore
Oct 10, 1998·Kidney International·J G UmansM E Wylam

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